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恶性疟原虫的聚合酶链反应及菌株鉴定

PCR and strain identification in Plasmodium falciparum.

作者信息

Wooden J, Kyes S, Sibley C H

机构信息

Department of Genetics (SK-50), University of Washington, Seattle, WA 98195, USA.

出版信息

Parasitol Today. 1993 Aug;9(8):303-5. doi: 10.1016/0169-4758(93)90131-x.

Abstract

Anyone who has cultured Plasmodium falciparum is aware that confusion about the identity of commonly used strains, and inadvertent contamination of one strain with another have been persistent problems. These issues have been recently reviewed by Robson and colleagues. Jason Wooden, Susan Kyes and Carol Hopkins Sibley have recently adapted two methods that offer a quick, easy alternative for the identification of P. falciparum strains in the laboratory.

摘要

任何培养过恶性疟原虫的人都知道,常用菌株的身份混淆以及一种菌株被另一种菌株意外污染一直是个问题。罗布森及其同事最近对这些问题进行了综述。贾森·伍德、苏珊·凯斯和卡罗尔·霍普金斯·西布利最近采用了两种方法,为实验室中鉴定恶性疟原虫菌株提供了一种快速、简便的替代方法。

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