从龙葵中分离出的糖蛋白可抑制核因子-κB（NF-κB）和激活蛋白-1（AP-1）的DNA结合活性，并增加佛波酯（TPA）刺激的MCF-7细胞中一氧化氮的生成。
Glycoprotein isolated from Solanum nigrum L. inhibits the DNA-binding activities of NF-kappaB and AP-1, and increases the production of nitric oxide in TPA-stimulated MCF-7 cells.
作者信息
Heo K S, Lee S J, Ko J H, Lim K, Lim K T
机构信息
521 Molecular Biochemistry Laboratory and Biodefensive Substances Group, Institute of Biotechnology, Chonnam National University, 300 Yongbong Dong, Bukgu, Kwangju 500757, South Korea.
出版信息
Toxicol In Vitro. 2004 Dec;18(6):755-63. doi: 10.1016/j.tiv.2004.03.003.
Solanum nigrum L. (SNL) has been used in traditional folk medicine to treat numerous cancers. We isolated a glycoprotein (150 kDa) from SNL and tested its effect on the modulation of transcriptional factors (NF-kappa B and AP-1) and iNO production in TPA induced-MCF-7 cells, which are part of the human breast cancer cell line, without estrogen receptors. However, the mechanism of SNL glycoprotein in pharmacological and biochemical actions in cancer cells has not been studied. To test the effect of SNL glycoprotein on the DNA-binding activities of nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1), and nitric oxide (NO) production, these experiments were carried out using electrophoretic mobility shift assays (EMSA), western blot analysis, and the Griess method. Results in this experiment showed that SNL glycoprotein inhibits 12-O-Tetra decanoylphorbol-13-acetate (TPA; 100 nM)-induced DNA-binding activities of NF-kappaB and AP-1, and enhances NO production in MCF-7 cells. That is, our results indicated that SNL glycoprotein has the capacity to modulate the TPA-induced DNA-binding activities of transcription factors and NO production, which play a critical role with respect to cytotoxicity in MCF-7 cells. Therefore, SNL glycoprotein might be one of the agents that blocks TPA-mediated signal responses in tumor cells.
龙葵已被用于传统民间医学治疗多种癌症。我们从龙葵中分离出一种糖蛋白（150 kDa），并测试了其对转录因子（NF-κB和AP-1）调节以及在佛波酯（TPA）诱导的MCF-7细胞（属于人乳腺癌细胞系且无雌激素受体）中诱导型一氧化氮合酶（iNO）产生的影响。然而，龙葵糖蛋白在癌细胞药理和生化作用中的机制尚未得到研究。为了测试龙葵糖蛋白对核因子-κB（NF-κB）和激活蛋白-1（AP-1）的DNA结合活性以及一氧化氮（NO）产生的影响，我们使用电泳迁移率变动分析（EMSA）、蛋白质免疫印迹分析和格里斯方法进行了这些实验。本实验结果表明，龙葵糖蛋白可抑制佛波酯（TPA；100 nM）诱导的NF-κB和AP-1的DNA结合活性，并增强MCF-7细胞中NO的产生。也就是说，我们的结果表明龙葵糖蛋白具有调节TPA诱导的转录因子DNA结合活性和NO产生的能力，而这在MCF-7细胞的细胞毒性方面起着关键作用。因此，龙葵糖蛋白可能是阻断肿瘤细胞中TPA介导信号反应的药物之一。
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