Rosa R, Costa P R, Pereira J, Nunes M L
Departamento de Inovação Tecnológica e Valorização dos Produtos da Pesca, IPIMAR, Avenida de Brasília, 1449-006 Lisboa, Portugal.
Comp Biochem Physiol B Biochem Mol Biol. 2004 Oct;139(2):299-310. doi: 10.1016/j.cbpc.2004.08.002.
The effect of spermatogenesis and oogenesis on protein, lipid, glycogen, cholesterol and energy contents, total amino acid and fatty acid profiles of Eledone cirrhosa and Eledone moschata tissues (gonad, digestive gland and muscle) was investigated. A significant (p<0.05) increase in the amino acids and protein content of the gonad throughout sexual maturation (namely in oogenesis) was observed, but the allocation of these nitrogen compounds from the digestive gland and muscle was not evident. The major essential amino acids (EAA) in the three tissues were leucine, lysine and arginine. The major nonessential amino acids (NEAA) were glutamic acid, aspartic acid and alanine. A significant increase in lipid and fatty acid contents of gonad and digestive gland was observed. There was also little evidence of accumulated lipid storage reserves being used for egg production. It seems that for egg production Eledone species use energy directly from food, rather than from stored products. Most of saturated fatty acid (SFA) content of the three tissues was presented as 16:0 and 18:0, monounsaturated fatty acid (MUFA) content as 18:1 and 20:1 and polyunsaturated fatty acid (PUFA) content as 20:4n-6, 20:5n-3 and 22:6n-3. Cholesterol and glycogen contents significantly increased in gonad and digestive gland throughout maturation while the muscle revealed no obvious pattern. If Eledone's component sterols are of a dietary origin, a considerable variation in the cholesterol content between species might be expected on the basis of the sterol composition of their prey. Although spermatogenesis and oogenesis had a significant effect (p<0.05) in gonad and digestive gland energy content, the biochemical composition of digestive gland and muscle may not be primarily influenced by sexual maturation, but rather by other biotic factors such as feeding activity, food availability, spawning and brooding.
研究了精子发生和卵子发生对卷须艾氏章鱼和麝香艾氏章鱼组织（性腺、消化腺和肌肉）中蛋白质、脂质、糖原、胆固醇和能量含量、总氨基酸和脂肪酸谱的影响。在整个性成熟过程中（即卵子发生过程中），观察到性腺中氨基酸和蛋白质含量显著（p<0.05）增加，但消化腺和肌肉中这些含氮化合物的分配不明显。这三种组织中的主要必需氨基酸（EAA）是亮氨酸、赖氨酸和精氨酸。主要的非必需氨基酸（NEAA）是谷氨酸、天冬氨酸和丙氨酸。观察到性腺和消化腺中脂质和脂肪酸含量显著增加。也几乎没有证据表明积累的脂质储存储备被用于产卵。似乎对于产卵，艾氏章鱼物种直接从食物中获取能量，而不是从储存产物中获取。这三种组织中大部分饱和脂肪酸（SFA）含量表现为16:0和18:0，单不饱和脂肪酸（MUFA）含量表现为18:1和20:1，多不饱和脂肪酸（PUFA）含量表现为20:4n-6、20:5n-3和22:6n-3。在整个成熟过程中，性腺和消化腺中的胆固醇和糖原含量显著增加，而肌肉则没有明显变化。如果艾氏章鱼的组成甾醇来自饮食，那么根据其猎物的甾醇组成，预计不同物种之间的胆固醇含量会有相当大的差异。虽然精子发生和卵子发生对性腺和消化腺的能量含量有显著影响（p<0.05），但消化腺和肌肉的生化组成可能主要不是受性成熟影响，而是受其他生物因素如摄食活动、食物可获得性、产卵和育雏的影响。
