Elson-Riggins Jocelyn G, Riggins Scott A, Gulland Frances M D, Platzer Edward G
Department of Biology, University of California, Riverside, California 92521, USA.
J Wildl Dis. 2004 Jul;40(3):466-75. doi: 10.7589/0090-3558-40.3.466.
Immunoglobulin (Ig) binding patterns of Pacific harbor seals (PHS, Phoca vitulina richardsi) and northern elephant seals (NES, Mirounga angustirostris) to tissues of adult Otostrongylus circumlitus were examined by immunoblotting to investigate the role of age in the unusual response of juvenile NES to infection with O. circumlitus. Serum was taken from NES between March 1997 and March 2001 and from PHS between May 1996 and August 1999. The serum of seals infected with O. circumlitus contained antibodies that bound to all nematode tissues examined. Intensity of band staining on Western blots suggested that there were higher levels of antibody recognizing the excretory-secretory (ES) glands in the serum of NES that were 1 yr and older and in the majority of PHS compared with that in 2- to 9-mo-old NES. All juvenile NES infected with O. circumlitus and a proportion of the PHS and older NES infected with O. circumlitus contained Ig specific to a 28 kDa protein band that was dominant in the female reproductive tract of the nematode. The Ig binding patterns of NES and PHS to adult Parafilaroides sp., larval Pseudoterranova sp., and larval and adult Anisakis sp. differed sufficiently from that of O. circumlitus that immunoblotting for the 28 kDa protein could be useful for diagnosis of this parasite in juvenile NES. The banding patterns suggest that O. circumlitus nematodes die and disintegrate in PHS and NES and that NES of 1 yr and older and most PHS respond differently to the ES glands than 2- to 9-mo-old NES.
通过免疫印迹法检测了太平洋斑海豹（PHS，Phoca vitulina richardsi）和北海象海豹（NES，Mirounga angustirostris）对成年环纹奥斯特线虫（Otostrongylus circumlitus）组织的免疫球蛋白（Ig）结合模式，以研究年龄在幼年NES对环纹奥斯特线虫感染异常反应中的作用。血清取自1997年3月至2001年3月的NES以及1996年5月至1999年8月的PHS。感染环纹奥斯特线虫的海豹血清中含有与所有检测的线虫组织结合的抗体。蛋白质印迹上条带染色强度表明，与2至9月龄的NES相比，1岁及以上的NES血清以及大多数PHS血清中识别排泄-分泌（ES）腺的抗体水平更高。所有感染环纹奥斯特线虫的幼年NES以及一部分感染环纹奥斯特线虫的PHS和成年NES都含有针对线虫雌性生殖道中占主导地位的一条28 kDa蛋白条带的特异性Ig。NES和PHS对成年副丝虫属（Parafilaroides sp.）、幼虫拟新地蛔属（Pseudoterranova sp.）以及幼虫和成年异尖线虫属（Anisakis sp.）的Ig结合模式与环纹奥斯特线虫的模式差异足够大，以至于针对28 kDa蛋白的免疫印迹可用于幼年NES中这种寄生虫的诊断。条带模式表明，环纹奥斯特线虫在PHS和NES中死亡并分解，1岁及以上的NES和大多数PHS对ES腺的反应与2至9月龄的NES不同。
