Bertelsen Mads F, Olberg Rolf-Arne, Crawshaw Graham J, Dibernardo Antonia, Lindsay L Robbin, Drebot Michael, Barker Ian K
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
J Wildl Dis. 2004 Jul;40(3):538-42. doi: 10.7589/0090-3558-40.3.538.
An outbreak of West Nile virus (WNV) infection occurred at a captive breeding facility for the endangered eastern loggerhead shrike (Lanius ludovicianus migrans) in August 2002. Within 10 d, five birds died; two were found dead, and the others died shortly after showing neurologic signs. West Nile virus was detected in all organs examined using immunohistochemistry, and its viral genome was amplified from brain and kidney samples using reverse transcription-polymerase chain reaction. None of the remaining birds in the colony had antibodies against WNV, which suggests a mortality rate of 100%. After vaccination with a commercial equine WNV vaccine 31 of 37 (84%) of the birds had WNV neutralizing antibodies.
2002年8月，西尼罗河病毒（WNV）在一家濒危东部 Loggerhead 伯劳（Lanius ludovicianus migrans）的圈养繁殖设施中爆发。在10天内，有5只鸟死亡；2只被发现死亡，其他几只在出现神经症状后不久死亡。使用免疫组织化学在所有检查的器官中检测到西尼罗河病毒，并使用逆转录-聚合酶链反应从脑和肾样本中扩增出其病毒基因组。该群体中其余的鸟均没有针对WNV的抗体，这表明死亡率为100%。在用商业马用WNV疫苗接种后，37只鸟中有31只（84%）产生了WNV中和抗体。
