Vielh P, Magdelénat H, Mosseri V, Remvikos Y, di Bonito L
Department of Pathology, Institut Curie, Paris, France.
Am J Clin Pathol. 1992 Feb;97(2):254-61. doi: 10.1093/ajcp/97.2.254.
In a prospective study of 50 consecutive fine-needle samples of breast cancer, determination of estrogen and progesterone receptors was performed by separate immunocytochemical and biochemical assays. Comparisons of immunocytochemical assays with biochemical assays were obtained in 42 cases (84%) for both steroid receptors and showed significant relationships for estrogen receptor (P less than 0.001, r = 0.85) and progesterone receptor (P less than 0.001, r = 0.89) when the percentage of labeled nuclei (immunocytochemical assay index) was considered. In addition, DNA flow cytometric analyses were performed on fine-needle samples in 34 cases, with measurable DNA ploidy and S-phase fraction in 33 (97%) and 31 cases (91%), respectively. These results show that (1) estrogen and progesterone receptor immunocytochemical assays are valuable tools for steroid receptor determination, and that (2) on the same fine-needle samples of breast cancer DNA flow cytometric analysis may be performed.
在一项对50例连续乳腺癌细针样本的前瞻性研究中，通过单独的免疫细胞化学和生化测定法对雌激素和孕激素受体进行了测定。在42例（84%）病例中，对两种类固醇受体的免疫细胞化学测定法与生化测定法进行了比较，当考虑标记细胞核的百分比（免疫细胞化学测定指数）时，雌激素受体（P<0.001，r = 0.85）和孕激素受体（P<0.001，r = 0.89）显示出显著相关性。此外，对34例细针样本进行了DNA流式细胞术分析，其中33例（97%）可测量DNA倍体，31例（91%）可测量S期分数。这些结果表明：（1）雌激素和孕激素受体免疫细胞化学测定法是测定类固醇受体的有价值工具；（2）在相同的乳腺癌细针样本上可进行DNA流式细胞术分析。
