Oishi Kazuhiko, Yoshizumi Tomokazu, Seki Noriyasu, Uchida Masaatsu K
Department of Pharmacology, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan.
Biol Pharm Bull. 2004 Oct;27(10):1544-8. doi: 10.1248/bpb.27.1544.
The changes in the reorganization of actin filaments during desensitization of secretion were investigated by transfecting RBL-2H3 cells with cDNA encoding the human m3 muscarinic acetylcholine receptors (RBL-m3 cells). Incubation of RBL-m3 cells with 10-100 microM carbachol in Ca2+ -free medium developed membrane ruffling. When the cells were desensitized under the condition where desensitization of carbachol-induced secretion occurred, desensitized cells failed to develop membrane ruffling with the subsequent addition of carbachol. These results suggest that m3 muscarinic receptor-mediated desensitization of secretion involves negative regulation of actin reorganization leading to membrane ruffling.
通过用编码人m3型毒蕈碱型乙酰胆碱受体的cDNA转染RBL-2H3细胞（RBL-m3细胞），研究了分泌脱敏过程中肌动蛋白丝重组的变化。在无钙培养基中用10-100微摩尔卡巴胆碱孵育RBL-m3细胞会产生膜皱襞。当细胞在卡巴胆碱诱导的分泌脱敏发生的条件下进行脱敏时，脱敏后的细胞在随后加入卡巴胆碱时未能产生膜皱襞。这些结果表明，m3型毒蕈碱受体介导的分泌脱敏涉及对导致膜皱襞的肌动蛋白重组的负调控。
