Uemura Kazuhide, Yamamoto Harumi, Nakagawa Tomoaki, Nakamura Kishiko, Kawasaki Nobuko, Oka Shogo, Ma Bruce Yong, Kawasaki Toshisuke
Department of Biological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501 Japan.
Glycoconj J. 2004;21(1-2):79-84. doi: 10.1023/B:GLYC.0000043753.11090.88.
Mannan-binding protein (MBP) is a Ca(2+)-dependent mammalian lectin that plays an important role in innate immunity. In this study, we found that ligand-bound MBP stimulates polymorphonuclear leukocytes (PMN) to induce cell aggregation and superoxide production. The biological response of PMN to ligand-bound MBP was dose- and time-dependent. The PMN aggregation and superoxide production induced by ligand-bound MBP was blocked completely by pertussis toxin, and partially blocked by a platelet activation factor receptor antagonist, TCV-309. These findings suggest that the ligand-bound MBP stimulates PMN through a putative MBP receptor(s) on PMN.
甘露聚糖结合蛋白（MBP）是一种依赖钙离子的哺乳动物凝集素，在天然免疫中起重要作用。在本研究中，我们发现配体结合的MBP刺激多形核白细胞（PMN）诱导细胞聚集和超氧化物生成。PMN对配体结合的MBP的生物学反应呈剂量和时间依赖性。配体结合的MBP诱导的PMN聚集和超氧化物生成被百日咳毒素完全阻断，并被血小板活化因子受体拮抗剂TCV-309部分阻断。这些发现表明，配体结合的MBP通过PMN上假定的MBP受体刺激PMN。
