Aguilar María R, Gallardo Alberto, Lechuga Laura M, Calle Ana, San Román Julio
Departamento de Química Macromolecular, Instituto de Ciencia y Tecnología de Polímeros, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain.
Macromol Biosci. 2004 Jul 14;4(7):631-8. doi: 10.1002/mabi.200400039.
The interpolyelectrolyte complex formation between chitosan and anionic polyacrylic derivatives, bearing sulfonic moieties, as well as the protein adsorption onto the chitosan/polyacrylic complexes were studied by surface plasmon resonance (SPR) optical biosensor. This unique technique allows a real time monitoring of different surface molecular interactions with very high sensitivity. The acrylic macromolecules are two families of copolymers of 2-acrylamido-2-methylpropane sulfonic acid (AMPS) and, respectively, 2-hydroxyethylmethacrylate (HEMA) and N,N'-dimethylacrylamide (DMAA). The complexation process was evaluated through the SPR measurements resulting from the flowing of polyacrylic aqueous solution over the sensor previously coated with chitosan. The SPR was able to differentiate strong ionic bonds from other weak and reversible interactions. By means of the coated sensors (uncomplexed and the whole series of complexed chitosan), SPR cold be used for a simple "in vitro" protein adsorption analysis, by flowing aqueous solutions of albumin and fibrinogen. While both proteins were adsorbed on the uncomplexed chitosan, the complexed coatings exhibited different and very promising behaviors. In particular, they showed no adsorption or only selective adsorption of albumin.
通过表面等离子体共振（SPR）光学生物传感器研究了壳聚糖与带有磺酸基团的阴离子聚丙烯酸衍生物之间的聚电解质复合物形成，以及蛋白质在壳聚糖/聚丙烯酸复合物上的吸附。这种独特的技术能够以非常高的灵敏度实时监测不同的表面分子相互作用。丙烯酸大分子是2-丙烯酰胺基-2-甲基丙烷磺酸（AMPS）分别与甲基丙烯酸2-羟乙酯（HEMA）和N，N'-二甲基丙烯酰胺（DMAA）的两类共聚物。通过将聚丙烯酸水溶液流过预先涂覆有壳聚糖的传感器所产生的SPR测量来评估络合过程。SPR能够区分强离子键与其他弱的和可逆的相互作用。借助于涂覆的传感器（未络合的以及整个系列的络合壳聚糖），通过使白蛋白和纤维蛋白原的水溶液流过，SPR可用于简单的“体外”蛋白质吸附分析。虽然两种蛋白质都吸附在未络合的壳聚糖上，但络合涂层表现出不同且非常有前景的行为。特别是，它们对白蛋白没有吸附或仅表现出选择性吸附。
