Neumann Thorsten, Haupt Björn, Ballauff Matthias
Polymer-Institute, University of Karlsruhe, Kaiserstrasse 12, 76128 Karlsruhe, Germany.
Macromol Biosci. 2004 Jan 21;4(1):13-6. doi: 10.1002/mabi.200300053.
We present a novel type of nanoreactor suitable for the immobilization of enzymes. The particles used consist of a polystyrene core onto which long chains of poly(acrylic acid) are grafted ("spherical polyelectrolyte brush"). Proteins adsorbed spontaneously onto these particles from aqueous solutions if the ionic strength is low. We immobilized glucoamylase on these particles and showed that this enzyme keeps nearly its full activity. This is shown by analyzing the enzymatic activity in terms of the Michaelis-Menten kinetics. No leaching out of the enzyme takes place during the reaction and the colloidal stability is not impeded by the adsorbed biomolecules. The data presented here suggest that the principle of immobilizing enzymes on these particles may be of general use.The Figure shows a schematic representation of the colloidal nanoreactors.
我们展示了一种适用于固定化酶的新型纳米反应器。所使用的颗粒由聚苯乙烯核组成，在其上面接枝了聚丙烯酸长链（“球形聚电解质刷”）。如果离子强度较低，蛋白质会从水溶液中自发吸附到这些颗粒上。我们将葡糖淀粉酶固定在这些颗粒上，并表明这种酶几乎保持其全部活性。这通过根据米氏动力学分析酶活性得以证明。在反应过程中酶不会浸出，并且吸附的生物分子不会妨碍胶体稳定性。此处呈现的数据表明，将酶固定在这些颗粒上的原理可能具有普遍用途。图展示了胶体纳米反应器的示意图。
