Zhou Shaobing, Liao Xueyan, Liang Zhenglun, Li Xiaohong, Deng Xianmo, Li Heming
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, P. O. Box 415, China.
Macromol Biosci. 2004 Jan 21;4(1):47-52. doi: 10.1002/mabi.200300044.
Poly-DL-lactide-poly(ethylene glycol) (PELA) microspheres containing Hepatitis B surface antigen (HBsAg) were elaborated by a solvent extraction method based on the formation of a double water/oil/water (w/o/w) emulsion. Microspheres were characterized in terms of morphology, size and size distribution, encapsulation efficiency, and the efficiency of microsphere formation (EMF). Transmission electron microscopy (TEM) and polyacrylamide gel electrophoresis (PAGE) were used to investigate the structural integrality of HBsAg encapsulated in PELA microspheres. The release profile was investigated by the measurement of antigen present in the release medium at various intervals. The PELA-10 microspheres displayed the highest antigen encapsulation efficiency (about 80%), and antigen molecules could be stabilized in the PELA-10 microspheres during the preparation process. It suggested that the PELA microspheres had a great potential as a new polymer adjuvant for HBsAg. The release of Hepatitis B surface antigen from poly-DL-lactide-poly(ethylene glycol) microspheres.
通过基于双水/油/水（w/o/w）乳液形成的溶剂萃取法制备了含有乙型肝炎表面抗原（HBsAg）的聚-DL-丙交酯-聚（乙二醇）（PELA）微球。对微球进行了形态、大小和大小分布、包封效率以及微球形成效率（EMF）方面的表征。采用透射电子显微镜（TEM）和聚丙烯酰胺凝胶电泳（PAGE）研究了包裹在PELA微球中的HBsAg的结构完整性。通过在不同时间间隔测量释放介质中存在的抗原，研究了释放曲线。PELA-10微球显示出最高的抗原包封效率（约80%），并且在制备过程中抗原分子可在PELA-10微球中稳定存在。这表明PELA微球作为HBsAg的新型聚合物佐剂具有很大潜力。乙型肝炎表面抗原从聚-DL-丙交酯-聚（乙二醇）微球中的释放。
