• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

通过根癌农杆菌将甜菜碱醛脱氢酶基因转化的耐盐白三叶

[Saline tolerance white clover transformed with the betaine aldehyde dehyrogenase gene by Agrobacterium tumefaciens].

作者信息

Chen Chuan-Fang, Li Yi-Wen, Chen Yu, Bai Jian-Rong, Li Hui, Zhu Yin-Feng, Chen Shou-Yi, Jia Xu

机构信息

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

出版信息

Yi Chuan Xue Bao. 2004 Jan;31(1):97-101.

PMID:15468926
Abstract

The white clover has been transformed with the Betaine Aldehyde Dehydrogenase (BADH) gene cloned from Atriplex hortensis by Agrobacterium tumefaciens. The relative electronic conductivity of the transgenic plants under 1% NaCl stress for 48 hours was about 20%, less than the control plant's relative electronic conductivity (more than 40%), these showed the cell membrane of the transgenic plants has been less injured than control plants under salt stress. The other experience showed that the transgenic plant could grow well in water culture included 0.5% NaCl for more than two weeks, but the control plants could not.

摘要

通过根癌农杆菌将从藜科植物中克隆得到的甜菜碱醛脱氢酶(BADH)基因导入白三叶草。在1%氯化钠胁迫48小时后,转基因植株的相对电导率约为20%,低于对照植株(超过40%),这表明在盐胁迫下转基因植株的细胞膜损伤程度小于对照植株。另一实验表明,转基因植株能够在含有0.5%氯化钠的水培条件下生长两周以上,而对照植株则不能。

相似文献

1
[Saline tolerance white clover transformed with the betaine aldehyde dehyrogenase gene by Agrobacterium tumefaciens].通过根癌农杆菌将甜菜碱醛脱氢酶基因转化的耐盐白三叶
Yi Chuan Xue Bao. 2004 Jan;31(1):97-101.
2
Enhanced stress tolerance in Escherichia coli and Nicotiana tabacum expressing a betaine aldehyde dehydrogenase/choline dehydrogenase fusion protein.表达甜菜碱醛脱氢酶/胆碱脱氢酶融合蛋白的大肠杆菌和烟草中增强的胁迫耐受性。
Biotechnol Prog. 2002 Nov-Dec;18(6):1176-82. doi: 10.1021/bp020057k.
3
[The transformation of betA gene into the pollen plantlets of Populus simonii x P. nigra].[将betA基因导入小叶杨×黑杨花粉植株]
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Apr;32(2):163-8.
4
[Production of transgenic rape plants (Brassica napus L.) using Agrobacterium tumefaciens].利用根癌农杆菌生产转基因油菜植株(甘蓝型油菜)
Genetika. 2000 Jul;36(7):932-41.
5
[Overexpression of Spinacia oleracea betaine aldehyde dehydrogenase (SoBADH) gene confers the salt and cold tolerant in Gossypium hirsutum L].菠菜甜菜碱醛脱氢酶(SoBADH)基因的过表达赋予陆地棉耐盐和耐寒性
Sheng Wu Gong Cheng Xue Bao. 2008 Aug;24(8):1464-9.
6
HAL1 mediate salt adaptation in Arabidopsis thaliana.HAL1介导拟南芥的盐适应性。
Cell Res. 2001 Jun;11(2):142-8. doi: 10.1038/sj.cr.7290079.
7
Improved salt tolerance in tobacco plants by co-transformation of a betaine synthesis gene BADH and a vacuolar Na+/H+ antiporter gene SeNHX1.通过共转化甜菜碱合成基因BADH和液泡Na+/H+逆向转运蛋白基因SeNHX1提高烟草植株的耐盐性。
Biotechnol Lett. 2008 Feb;30(2):369-76. doi: 10.1007/s10529-007-9548-6. Epub 2007 Oct 30.
8
Plastid-expressed betaine aldehyde dehydrogenase gene in carrot cultured cells, roots, and leaves confers enhanced salt tolerance.在胡萝卜培养细胞、根和叶中质体表达的甜菜碱醛脱氢酶基因赋予了增强的耐盐性。
Plant Physiol. 2004 Sep;136(1):2843-54. doi: 10.1104/pp.104.045187. Epub 2004 Sep 3.
9
Expression of the spinach betaine aldehyde dehydrogenase (BADH) gene in transgenic tobacco plants.菠菜甜菜碱醛脱氢酶(BADH)基因在转基因烟草植株中的表达。
Chin J Biotechnol. 1997;13(3):153-9.
10
Highly efficient transformation and plant regeneration of tall fescue mediated by Agrobacterium tumefaciens.根癌农杆菌介导的高羊茅高效转化与植株再生
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2005 Apr;31(2):149-59.