[三种用于快速筛查糙小麦中脱氧雪腐镰刀菌烯醇的商用酶联免疫吸附测定试剂盒的评估]

[Evaluation of three commercial ELISA kits for rapid screening of deoxynivalenol in unpolished wheat].

作者信息

Sugita-Konishi Yoshiko, Takatori Kosuke, Tanaka Toshitsugu, Sugiura Yoshitsugu, Nakajima Masahiro, Tanaka Kenji, Kumagai Susumu

机构信息

National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.

出版信息

Shokuhin Eiseigaku Zasshi. 2004 Jun;45(3):156-60. doi: 10.3358/shokueishi.45.156.

DOI:10.3358/shokueishi.45.156

PMID:15468936

Abstract

Evaluation of commercial ELISA kits for the screening of deoxynivalenol (DON) was carried out. Three kinds of commercial kits supplied by different companies were used. Three lots of naturally contaminated wheat and DON-free wheat (<0.05 microg/g) were used as samples. The values obtained from ELISA were compared with those of the HPLC-UV method. In the results obtained from 14 institutions using ELISA, the CV values were less than 17.6% for all the commercial kits. The DON-free sample was not detected as positive with any commercial kits. Also there was no negative finding among the naturally contaminated samples used in this experiment. Coefficients of determination in ELISA and HPLC analysis were 0.979-0.999. These results indicate that ELISA using any of the three commercial kits is efficient for the screening of DON in wheat.

摘要

对用于筛选脱氧雪腐镰刀菌烯醇（DON）的商用酶联免疫吸附测定（ELISA）试剂盒进行了评估。使用了不同公司提供的三种商用试剂盒。三批天然污染小麦和不含DON的小麦（<0.05微克/克）用作样本。将ELISA获得的值与高效液相色谱-紫外检测法（HPLC-UV）的值进行比较。在14个机构使用ELISA获得的结果中，所有商用试剂盒的变异系数（CV）值均小于17.6%。不含DON的样本用任何商用试剂盒检测均未呈阳性。此外，在本实验中使用的天然污染样本中也没有出现阴性结果。ELISA和HPLC分析的决定系数为0.979 - 0.999。这些结果表明，使用这三种商用试剂盒中的任何一种进行ELISA都能有效地筛选小麦中的DON。