Oboznaia M B, Vol'pina O M, Zhmak M N, Titova M A, Volkova T D, Egorov A A, Rybakov S S, Ivanov V T
Bioorg Khim. 2004 Jul-Aug;30(4):356-63.
The antibodies to the bovine prion protein were produced by immunizing mice of three lines with five synthetic fragments of the protein and their six analogues. The analogues contained the amino acid substitutions that, according to theoretical calculation, should lead to an increase in the immunogenic activity of peptides. All the peptides, except for one, induced the formation of antibodies. All the sera containing the antipeptide antibodies were tested by an immunohistochemical method. The sera that were effectively bound to the brain preparations from the bovine with spongiform encephalopathy were identified; it was shown that they do not interact with the preparations of normal brain. Therefore, it was shown that the immunization of mice with the synthetic fragments of a prion protein helps obtain specific antibodies suitable for the study and diagnostics of prion diseases.
通过用牛朊病毒蛋白的五个合成片段及其六个类似物免疫三个品系的小鼠，制备了抗牛朊病毒蛋白的抗体。这些类似物含有氨基酸取代，根据理论计算，这些取代应导致肽的免疫原活性增加。除了一个片段外，所有肽都诱导了抗体的形成。所有含有抗肽抗体的血清都通过免疫组织化学方法进行了检测。鉴定出了与患海绵状脑病的牛的脑制剂有效结合的血清；结果表明，它们不与正常脑的制剂相互作用。因此，已表明用朊病毒蛋白的合成片段免疫小鼠有助于获得适用于朊病毒疾病研究和诊断的特异性抗体。
