Li Mei-Hua, Yang Zhan-Quan, Yin Wan-Zhong
Department of Otorhinolaryngology Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun 130031, China.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2004 Jun;39(6):353-5.
To study the influence of the inhibitor of protein kinase C (PKC), Iso-H7 [1-(5-lsoquinolinyl-sulfony)-3-methylpiperazine dihydrochloride] on eosinophil apoptosis in nasal polyp tissues.
Nasal polyp tissues were cultured and divided into two groups, test group (with Iso-H7 in different concentration) and control group (without the inhibitor of PKC), to check out the influence of Iso-H7 on eosinophil apoptosis in nasal polyps. The nasal polyp tissues were cultured in Iso-H7 in concentration of 10(-6), 10(-4), 10(-2) mol/L for 1 d, 3 d, and 5 d, respectively. Eosinophil apoptosis index was measured with TUNEL (terminal-deoxynucleotidyl transferase d-UTP nick end labeling), in situ hybridization and MGG staining to identify eosinophil apoptosis.
Occasionally, there was little eosinophil apoptosis in two groups in 1 d, but in 3 d, There were a great deal of eosinophil apoptosis in test group. The eosinophil apoptosis indexs of the two groups were significantly different (P < 0.01). The indexs of eosinophil apoptosis was not significantly higher in 3 d than that in 5 d in test groups (P > 0. 05), but significantly higher than that in 1d (P < 0. 01).
Iso-H7 takes a important role in accelerating eosinophil apoptosis. The inhibitor of PKC could significantly promote eosinophil apoptosis in nasal polyps. There is no dosage and time dependence of PKC inhibitor in regulating eosinophil apoptosis of nasal polyps.
研究蛋白激酶C（PKC）抑制剂异搏定H7[1-（5-异喹啉磺酰基）-3-甲基哌嗪二盐酸盐]对鼻息肉组织中嗜酸性粒细胞凋亡的影响。
培养鼻息肉组织并分为两组，试验组（加入不同浓度的异搏定H7）和对照组（不加PKC抑制剂），以观察异搏定H7对鼻息肉中嗜酸性粒细胞凋亡的影响。将鼻息肉组织分别在浓度为10^(-6)、10^(-4)、10^(-2)mol/L的异搏定H7中培养1天、3天和5天。采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL）、原位杂交和MGG染色检测嗜酸性粒细胞凋亡指数，以鉴定嗜酸性粒细胞凋亡情况。
1天时两组中偶尔有少量嗜酸性粒细胞凋亡，但3天时试验组有大量嗜酸性粒细胞凋亡。两组嗜酸性粒细胞凋亡指数差异有统计学意义（P<0.01）。试验组3天时嗜酸性粒细胞凋亡指数与5天时相比差异无统计学意义（P>0.05），但显著高于1天时（P<0.01）。
异搏定H7在加速嗜酸性粒细胞凋亡中起重要作用。PKC抑制剂可显著促进鼻息肉中嗜酸性粒细胞凋亡。PKC抑制剂在调节鼻息肉嗜酸性粒细胞凋亡方面不存在剂量和时间依赖性。
