Cai Wen-Jie, Zhu Yi-Chun
Department of Physiology & Pathophysiology, Medical Center of Fudan University, Shanghai 200032.
Sheng Li Ke Xue Jin Zhan. 2004 Jul;35(3):205-9.
Embryonic stem cells are pluripotent cells derived from blastocyst-stage embryos. They are characterized by their capacity for self-renewal and by the ability to differentiate into a variety of cell types. In vitro, ES cells can spontaneously aggregate into the formation of embryoid bodies (EBs) comprised of a range of differentiated cells. When injected into severe combined immunodeficient (SCID) mice, ES cells will induce the generation of teratomas, which include differentiated cells from three embryonic germ layers. We can induce the differentiation of ES cells into cardiocytes by treatment with several growth factors or co-culture with certain other cells. The pure differentiated cardiocytes from ES cells are then selected and implanted into the infarcted hearts. The hearts' function will be improved. ES cells implantation is a novel and potential method to treat infarcted hearts.
胚胎干细胞是源自囊胚期胚胎的多能细胞。它们的特点是具有自我更新能力以及分化为多种细胞类型的能力。在体外，胚胎干细胞可自发聚集形成由一系列分化细胞组成的胚状体（EBs）。当注射到严重联合免疫缺陷（SCID）小鼠体内时，胚胎干细胞会诱导畸胎瘤的产生，其中包括来自三个胚胎胚层的分化细胞。我们可以通过用几种生长因子处理或与某些其他细胞共培养来诱导胚胎干细胞分化为心肌细胞。然后从胚胎干细胞中筛选出纯的分化心肌细胞并植入梗死心脏。心脏功能将得到改善。胚胎干细胞植入是一种治疗梗死心脏的新颖且有潜力的方法。
