Peng Xu, Kessler Alexandra, Phan Hien, Garrett Roger A, Prangishvili David
Danish Archaea Centre, Institute of Molecular Biology, Copenhagen University, Sølvgade 83H, DK-1307 Copenhagen K, Denmark.
Mol Microbiol. 2004 Oct;54(2):366-75. doi: 10.1111/j.1365-2958.2004.04287.x.
The DNA rudivirus SIRV1 of the hyperthermophilic archaeon Sulfolobus shows exceptional properties. Viral isolates invariably contain a population of variants with different but closely related genomes. Upon propagation in a given host strain, one or more genomes dominate in the viral population. However, upon passage into a new host strain the viral population undergoes changes and other dominant variants are selected. Sequencing and analysis of the variant genomes revealed that major differences occur in gene order, gene size and gene content at localized genomic sites. A previously unknown mechanism of genomic rearrangement involving putative 12 bp archaeal introns appears to facilitate alteration of the variant genomes. Inter-genomic recombination between the different variants also occurs. The variant genomes exhibit signature tetranucleotide sequences near their putative sites for replication initiation.
嗜热古菌硫磺矿硫化叶菌的DNA rudivirus病毒SIRV1具有特殊性质。病毒分离株总是包含一群具有不同但密切相关基因组的变体。在给定宿主菌株中繁殖时，一个或多个基因组在病毒群体中占主导地位。然而，当传代到新的宿主菌株时，病毒群体发生变化并选择其他优势变体。对变体基因组的测序和分析表明，主要差异发生在局部基因组位点的基因顺序、基因大小和基因含量上。一种涉及假定的12 bp古菌内含子的先前未知的基因组重排机制似乎促进了变体基因组的改变。不同变体之间也发生基因组间重组。变体基因组在其假定的复制起始位点附近表现出特征性的四核苷酸序列。
