Alexandrakis Michael G, Passam Freda H, Sfiridaki Katerina, Kafarakis Panayiotis, Karydi Elli, Liapi Dimitra, Perisinakis Kostas, Kyriakou Despina S
Department of Haematology, Division of Medicine, Medical School of Crete, P.O. Box 1352, Heraklion, Crete, Greece.
Clin Chim Acta. 2004 Nov;349(1-2):39-43. doi: 10.1016/j.cccn.2004.05.025.
The expression of adhesion molecules is important for the interaction of myeloma cells with the bone marrow microenvironment. In the current study, serum soluble adhesion molecules (sICAM-1 and sE-selectin) were measured in untreated multiple myeloma (MM) patients in relation with other markers of disease activity.
The study group consisted of 67 patients with MM (classified according to the Durie-Salmon classification) and 15 controls. Interleukin-6 (IL-6), sICAM-1 and sE-selectin concentrations were determined by enzyme-linked immunosorbent assay (ELISA). In addition, the monoclonal protein, erythrocyte sedimentation rate (ESR) and hemoglobin (Hb) concentration were also determined.
Serum sICAM-1 level increased significantly at advanced stages of MM and was higher in comparison to controls (p<0.01). sE-selectin increased significantly with advancing stage of the disease, but did not differ from controls. IL-6, ESR and M-component were significantly higher and Hb concentrations lower with advancing stage of disease. There was a positive correlation of IL-6 with sICAM-1 and sE-selectin.
We conclude that serum sICAM-1 differs in multiple myeloma patients from normals and together with sE-selectin increase in parallel to increasing stage of disease, which may reflect a dysregulation and possible involvement of these adhesion molecules in myeloma progression.
黏附分子的表达对于骨髓瘤细胞与骨髓微环境的相互作用至关重要。在本研究中，检测了未经治疗的多发性骨髓瘤（MM）患者血清中的可溶性黏附分子（sICAM - 1和sE - 选择素），并与疾病活动的其他标志物进行关联分析。
研究组由67例MM患者（根据Durie - Salmon分类法进行分类）和15例对照组成。采用酶联免疫吸附测定（ELISA）法测定白细胞介素 - 6（IL - 6）、sICAM - 1和sE - 选择素的浓度。此外，还测定了单克隆蛋白、红细胞沉降率（ESR）和血红蛋白（Hb）浓度。
MM晚期患者血清sICAM - 1水平显著升高，与对照组相比更高（p<0.01）。sE - 选择素随疾病进展显著升高，但与对照组无差异。随着疾病进展，IL - 6、ESR和M成分显著升高，Hb浓度降低。IL - 6与sICAM - 1和sE - 选择素呈正相关。
我们得出结论，多发性骨髓瘤患者血清sICAM - 1与正常人不同，并且与sE - 选择素一起随着疾病分期的增加而升高，这可能反映了这些黏附分子的失调以及可能参与了骨髓瘤的进展。
