乙酰水杨酸抑制成年大鼠血小板的ATP二磷酸水解酶活性。

Acetylsalicylic acid inhibits ATP diphosphohydrolase activity by platelets from adult rats.

作者信息

Buffon Andréia, Ribeiro Vanessa B, Fürstenau Cristina R, Battastini Ana M O, Sarkis João J F

机构信息

Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600, ANEXO, CEP Porto Alegre, RS, Brazil.

出版信息

Clin Chim Acta. 2004 Nov;349(1-2):53-60. doi: 10.1016/j.cccn.2004.06.001.

DOI:10.1016/j.cccn.2004.06.001

PMID:15469855

Abstract

BACKGROUND AND METHODS

The in vitro effect of the nonsteroidal anti-inflammatory drug, acetylsalicylic acid (ASA), on the extracellular adenine nucleotide hydrolysis by intact rat blood platelets was studied.

RESULTS

Our results demonstrate that aspirin, at final concentrations of 2.0 and 3.0 mM, inhibits ATP extracellular hydrolysis in vitro by approximately 17% and 21%, respectively. Aspirin, at a final concentration of 3.0 mM, also inhibited in vitro extracellular ADP hydrolysis by approximately 41%. The same concentrations of this drug, however, did not alter AMP hydrolysis by intact rat blood platelets under similar assay conditions. The kinetic analysis demonstrated that the inhibition of ADP and ATP hydrolysis by aspirin in rat platelets is of the uncompetitive type.

CONCLUSION

In this study, we demonstrated an inhibitory effect of ASA upon E-NTPDase 3 activity of platelets from adult rats and discussed the significance of our findings.

摘要

背景与方法

研究了非甾体抗炎药乙酰水杨酸（ASA）对完整大鼠血小板细胞外腺嘌呤核苷酸水解的体外作用。

结果

我们的结果表明，最终浓度为2.0和3.0 mM的阿司匹林分别在体外抑制ATP细胞外水解约17%和21%。最终浓度为3.0 mM的阿司匹林在体外也抑制细胞外ADP水解约41%。然而，在相似的测定条件下，相同浓度的该药物并未改变完整大鼠血小板对AMP的水解。动力学分析表明，阿司匹林对大鼠血小板中ADP和ATP水解的抑制作用为非竞争性类型。