Abraham Premila
Department of Biochemistry, Christian Medical College, Bagayam, Vellore-632 002 Tamil Nadu, India.
Clin Chim Acta. 2004 Nov;349(1-2):61-5. doi: 10.1016/j.cccn.2004.06.002.
Biotinidase, an enzyme that is synthesized by the liver and secreted into the blood, decreases in plasma in both humans and experimental animal liver cirrhosis. In overdose conditions, paracetamol is known to cause centrilobular necrosis in both humans and experimental animals. We determined if there is any alteration in the activity of the enzyme in the plasma and liver of rats after subtoxic and toxic doses of paracetamol.
After 4-, 24-, and 40-h treatment of rats with either subtoxic (350 mg/kg body wt) or toxic dose (1000 mg per kg) of paracetamol intraperitoneally, biotinidase activity was assayed in the liver and plasma along with the albumin concentration and ALT activity.
After the subtoxic dose of paracetamol, there were no significant change in plasma biotinidase activity and liver biotinidase activity was observed at any time period after treatment. However, 24 and 40 h after the toxic dose of paracetamol, biotinidase activity was decreased in the liver and increased in the plasma as compared with the control, when plasma ALT was increased.
The increase in plasma biotinidase activity may serve as an indicator of paracetamol-induced acute liver injury in the rat.
生物素酶是一种由肝脏合成并分泌到血液中的酶，在人类和实验性动物肝硬化中，其血浆水平会降低。在过量用药的情况下，已知对乙酰氨基酚会在人类和实验动物中导致小叶中心坏死。我们测定了给予大鼠亚毒性和毒性剂量的对乙酰氨基酚后，其血浆和肝脏中该酶的活性是否有任何变化。
用亚毒性剂量（350毫克/千克体重）或毒性剂量（1000毫克/千克）的对乙酰氨基酚腹腔注射大鼠4小时、24小时和40小时后，测定肝脏和血浆中的生物素酶活性，同时测定白蛋白浓度和谷丙转氨酶活性。
给予亚毒性剂量的对乙酰氨基酚后，血浆生物素酶活性无显著变化，且在治疗后的任何时间段肝脏生物素酶活性均未观察到变化。然而，给予毒性剂量的对乙酰氨基酚24小时和40小时后，与对照组相比，肝脏中的生物素酶活性降低，血浆中的生物素酶活性升高，此时血浆谷丙转氨酶升高。
血浆生物素酶活性的升高可能作为大鼠对乙酰氨基酚诱导的急性肝损伤的一个指标。
