Demuth Karine, Ducros Véronique, Michelsohn Samantha, Paul Jean-Louis
Laboratoire de Biochimie Cardiovasculaire, Hôpital Européen Georges Pompidou, AP-HP, 75015 Paris, France.
Clin Chim Acta. 2004 Nov;349(1-2):113-20. doi: 10.1016/j.cccn.2004.06.012.
A lot of methods are now available for total plasma homocysteine (tHcy) determination. HPLC with fluorescence detection were the most widely used methods until recently but immunoassays, easier to use, currently supplant in-house laboratory methods.
The latest commercial automated chemiluminescence immunoassay (ICL) for tHcy, developed on the ADVIA Centaur analyzer (Bayer Diagnostics), was fully evaluated and compared with a current HPLC method.
The ICL-ADVIA Centaur method had a detection limit <0.9 micromol/l and was linear for tHcy between 2.4 and 58.8 micromol/l. The within- and between assay imprecision was <6% and <7%, respectively. The analytical recovery ranged from 93.5% to 109.7%. The comparison of 168 clinical plasma specimens indicated a good correlation between the two methods (r=0.96, p<0.0001) but a systematic positive bias for ICL-ADVIA Centaur method (mean difference=3.0 micromol/l).
The ADVIA Centaur method is a useful and practicable alternative to HPLC methods for tHcy determination. The ICL method is even more convenient than chromatographic methods for routine use because of its significantly higher throughput (200 samples/h vs. 40 per 24 h). However, the agreement among methods is insufficient to allow them to be used interchangeably.
目前有多种方法可用于测定血浆总同型半胱氨酸（tHcy）。直到最近，带有荧光检测的高效液相色谱法（HPLC）是最广泛使用的方法，但免疫测定法使用更简便，目前已取代了内部实验室方法。
对在ADVIA Centaur分析仪（拜耳诊断公司）上开发的最新商用tHcy自动化化学发光免疫测定法（ICL）进行了全面评估，并与当前的HPLC方法进行了比较。
ICL-ADVIA Centaur方法的检测限<0.9微摩尔/升，tHcy在2.4至58.8微摩尔/升之间呈线性。批内和批间不精密度分别<6%和<7%。分析回收率在93.5%至109.7%之间。对168份临床血浆标本的比较表明，两种方法之间具有良好的相关性（r=0.96，p<0.0001），但ICL-ADVIA Centaur方法存在系统性正偏差（平均差异=3.0微摩尔/升）。
ADVIA Centaur方法是用于tHcy测定的HPLC方法的一种有用且可行的替代方法。由于其通量显著更高（每小时200个样本对每24小时40个），ICL方法在常规使用中比色谱方法更方便。然而，方法之间的一致性不足以允许它们互换使用。
