Yang Hui Ming, Lee Psyche Huu-Hoa, Lim Tit Meng, Sheu Fwu-Shan
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
Brain Res Mol Brain Res. 2004 Oct 22;129(1-2):171-8. doi: 10.1016/j.molbrainres.2004.06.039.
To test a cellular effect of rodent neurogranin (Ng) oxidation as compared to Ng phosphorylation, we develop a cell model capable of stable expression of Ng using the Tet-On system, and determine whether Ng oxidation regulates intracellular calcium level. Our results show that Ng oxidation by nitric oxide donor induces an increase of Ca(2+) in Ng-expressed cells as compared to the control cells without expressing Ng. These results suggest that Ng oxidation plays a significant role in intracellular Ca(2+) homeostasis, essential for the activated signaling networks in learning and memory.
为了测试与啮齿动物神经颗粒蛋白（Ng）磷酸化相比，Ng氧化的细胞效应，我们利用Tet-On系统开发了一种能够稳定表达Ng的细胞模型，并确定Ng氧化是否调节细胞内钙水平。我们的结果表明，与未表达Ng的对照细胞相比，一氧化氮供体引起的Ng氧化会导致表达Ng的细胞中[Ca(2+)]i增加。这些结果表明，Ng氧化在细胞内Ca(2+)稳态中起重要作用，而细胞内Ca(2+)稳态对于学习和记忆中激活的信号网络至关重要。
