Yau Joyce L W, Noble June, Chapman Karen E, Seckl Jonathan R
Endocrinology Unit, Molecular Medicine Centre, Western General Hospital, Edinburgh EH4 2XU, Scotland, UK.
Brain Res Mol Brain Res. 2004 Oct 22;129(1-2):189-92. doi: 10.1016/j.molbrainres.2004.06.033.
Adult rats were given antidepressant drugs orally. Fluoxetine, but not moclobemide, venlafaxine, tianeptine or desipramine, increased total glucocorticoid receptor (GR) mRNA in the hippocampus after 4 weeks. Further examination revealed that GR mRNA containing the brain-specific exon 1(7) was increased across all hippocampal subregions. In contrast, expression of the major exon 1(10) and another brain-specific exon 1(5)-containing GR mRNAs were unchanged. Tissue-specific first exon usage may contribute to the differential regulation of GR by fluoxetine in brain subregions.
给成年大鼠口服抗抑郁药物。4周后，氟西汀可增加海马体中总糖皮质激素受体（GR）mRNA的含量，而吗氯贝胺、文拉法辛、噻奈普汀或地昔帕明则无此作用。进一步检测发现，含有脑特异性外显子1(7)的GR mRNA在海马体所有亚区均增加。相比之下，主要外显子1(10)和另一种含有脑特异性外显子1(5)的GR mRNA的表达未发生变化。组织特异性第一外显子的使用可能导致氟西汀对脑亚区GR的差异调节。
