Lefter Liviu, Scripcaru Viorel, Sunamura Makoto, Furukawa Toru, Matsuno Seiki, Oshimura Mitsuo, Drug Vasile, Dragomir Cristian, Horii Akira
3rd Department of Surgery, St. Spiridon University Hospital, Iasi, Romania.
Rom J Gastroenterol. 2004 Sep;13(3):195-201.
Pancreatic cancer has a well-known reputation as one of the leading causes of cancer deaths worldwide. Thus, acquisition of efficient approaches and markers for accurate detection at the earlier stages of the disease should be prioritized. We have been focusing on tumor suppressor genes (TSGs) activity in pancreatic cancer to find effective methods for its genetic diagnosis and/or treatment. In this study, we utilized the technique of micro-cell-mediated chromosome transfer (MMCT) to introduce a normal copy of human chromosome 18 individually into some pancreatic cancer cells. Subsequently, the tumorigenic ability of the resulting hybrids was assessed in vitro and in vivo. In vitro growth of the hybrid clones was significantly delayed as compared to the parental cells. This was paralleled by the hybrid cells promotion of invasive carcinomas in nude mice at a significantly lower rate and with a longer latency than the parental tumor cells. This study provides evidence that MMCT is an efficient tool for screening of tumor suppressor activity in pancreatic cancer. The functional data emerging from this study bring into sharp relief the implication of chromosome 18 as a putative location for new TSG(s), yet to be identified in this region.
胰腺癌是全球癌症死亡的主要原因之一，这是众所周知的。因此，应优先获取在疾病早期进行准确检测的有效方法和标志物。我们一直专注于胰腺癌中肿瘤抑制基因（TSG）的活性，以寻找其基因诊断和/或治疗的有效方法。在本研究中，我们利用微细胞介导的染色体转移（MMCT）技术，将人类18号染色体的正常拷贝分别导入一些胰腺癌细胞中。随后，在体外和体内评估所得杂交细胞的致瘤能力。与亲代细胞相比，杂交克隆的体外生长明显延迟。与此同时，杂交细胞在裸鼠中促进侵袭性癌的发生率明显低于亲代肿瘤细胞，且潜伏期更长。本研究提供了证据，表明MMCT是筛选胰腺癌中肿瘤抑制活性的有效工具。这项研究得出的功能数据清楚地表明，18号染色体作为新的TSG（尚未在该区域鉴定）的假定位置具有重要意义。
