de Lima Maria Noêmia M, Laranja Daniela C, Bromberg Elke, Roesler Rafael, Schröder Nadja
Memory and Neurodegeneration Laboratory, Department of Physiological Sciences, Biosciences Faculty, Catholic University (PUCRS), 90619-900 Porto Alegre, RS, Brazil.
Behav Brain Res. 2005 Jan 6;156(1):139-43. doi: 10.1016/j.bbr.2004.05.016.
The aim of the present study was to investigate the effects of NMDA receptor blockade on formation of object recognition memory. In the first experiment, adult Wistar rats were given an intraperitoneal injection of saline or the NMDA receptor antagonist [(+)-5-methyl-10,11-dihydro-5H-dibenzo-[a,d]cyclo-hepten-5,10-imine-maleate] (MK-801) (0.001, 0.01, or 0.1 mg/kg) 20 min prior to training in a novel object recognition task. In the second experiment, saline or MK-801 (0.1 mg/kg) were given immediately after training. Memory retention was tested 1.5 and 24 h after training. MK-801 impaired both short- and long-term retention of object recognition memory when given either before or after training. The results suggest that NMDA receptor activation is necessary for formation of object recognition memory.
本研究的目的是探究N-甲基-D-天冬氨酸（NMDA）受体阻断对物体识别记忆形成的影响。在第一个实验中，成年Wistar大鼠在进行新物体识别任务训练前20分钟，腹腔注射生理盐水或NMDA受体拮抗剂[（+）-5-甲基-10,11-二氢-5H-二苯并[a,d]环庚烯-5,10-亚胺马来酸盐]（MK-801）（0.001、0.01或0.1毫克/千克）。在第二个实验中，训练后立即注射生理盐水或MK-801（0.1毫克/千克）。在训练后1.5小时和24小时测试记忆保持情况。无论在训练前还是训练后给予MK-801，均会损害物体识别记忆的短期和长期保持。结果表明，NMDA受体激活对于物体识别记忆的形成是必要的。
