Sasaki Hidefumi, Moriyama Satoru, Nakashima Yoshiaki, Kobayashi Yoshihiro, Kiriyama Masanobu, Fukai Ichiro, Yamakawa Yosuke, Fujii Yoshitaka
Department of Surgery II, Nagoya City University Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
Lung Cancer. 2004 Nov;46(2):171-8. doi: 10.1016/j.lungcan.2004.03.021.
Histone deacetylases (HDACs) play a crucial role in tumorigenesis, however, the expression status of HDACs in lung cancer tissues has not been reported. We have investigated that HIDAC 1 mRNA levels and other clinico-pathological data, including MTA 1 mRNA expression in lung cancer. The study included 102 lung cancer cases. The HDAC1 mRNA levels were quantified by real time reverse transcription-polymerase chain reaction (RT-PCR) using LightCycler (Roche Molecular Biochemicals, Mannheim, Germany). The HDAC1/GAPDH mRNA levels were not significantly different in tumor tissues from lung cancer (30.654 +/- 33.047) and adjacent non-malignant lung tissues (18.953 +/- 56.176 , P = 0.1827). No significant difference in HDAC1/GAPDH mRNA levels was found among age, gender, and lymph node metastasis. The HDAC1/GAPDH mRNA levels were significantly higher in stage III or IV lung cancer (50.929 +/- 120.433) than in stage I lung cancer (11.430 +/- 25.611, P = 0.0472). HDAC1/GAPDH mRNA levels were significantly higher in T3 or T4 lung carcinoma (54.326 +/- 127.018) than in T1 or T2 lung cancers (14.790 +/- 48.670, P = 0.1601). HDAC1/GAPDH mRNA levels were correlated with MTA1/GAPDH mRNA levels (y = 0.0106x + 2.5827 , P = 0.0352 ). HDAC1/GAPDH mRNA levels were also correlated with HDAC1 protein (P = 0.0484) expression by immunohistochemistry. Using the LightCycler RT-PCR assay, the HDAC1 gene expression might correlate with progression of lung cancers. However, further studies are needed to confirm the impact of HDAC1 for the molecular target of the lung cancer.
组蛋白去乙酰化酶（HDACs）在肿瘤发生过程中起着关键作用，然而，HDACs在肺癌组织中的表达状况尚未见报道。我们研究了肺癌中HIDAC 1 mRNA水平及其他临床病理数据，包括MTA 1 mRNA表达。该研究纳入了102例肺癌病例。使用罗氏LightCycler（德国曼海姆罗氏分子生化公司）通过实时逆转录聚合酶链反应（RT-PCR）对HDAC1 mRNA水平进行定量。肺癌肿瘤组织（30.654±33.047）与相邻非恶性肺组织（18.953±56.176，P = 0.1827）中的HDAC1/GAPDH mRNA水平无显著差异。在年龄、性别和淋巴结转移方面，HDAC1/GAPDH mRNA水平未发现显著差异。III期或IV期肺癌（50.929±120.433）中的HDAC1/GAPDH mRNA水平显著高于I期肺癌（11.430±25.611，P = 0.0472）。T3或T4期肺癌（54.326±127.018）中的HDAC1/GAPDH mRNA水平显著高于T1或T2期肺癌（14.790±48.670，P = 0.1601）。HDAC1/GAPDH mRNA水平与MTA1/GAPDH mRNA水平相关（y = 0.0106x + 2.5827，P = 0.0352）。通过免疫组织化学检测，HDAC1/GAPDH mRNA水平也与HDAC1蛋白表达相关（P = 0.0484）。使用LightCycler RT-PCR检测法，HDAC1基因表达可能与肺癌进展相关。然而，需要进一步研究来证实HDAC1对肺癌分子靶点的影响。
