海胆（光棘球海胆）卵中的类胰凝乳蛋白酶和类胰蛋白酶活性。

Chymotrypsin-like and trypsin-like protease activities in the sea urchin (Hemicentrotus pulcherrimus) egg.

作者信息

Taniguchi Y

机构信息

Zoological Institute, Faculty of Science, University of Tokyo, Japan.

出版信息

Experientia. 1992 Mar 15;48(3):287-90. doi: 10.1007/BF01930478.

DOI:10.1007/BF01930478

PMID:1547864

Abstract

Proteolytic activities in extracts of sea urchin eggs were examined using SDS (sodium dodecyl sulphate)-polyacrylamide gels. In the unfertilized eggs, proteases were detected as bands corresponding to the molecular weights of 40 kD and 26 kD on the gelatin gel, and 35 kD and 30 kD on the casein gel. Using various protease inhibitors, it was found that 40 kD, 30 kD, and 26 kD are chymotrypsin-like proteases and that 35 kD is a trypsin-like protease. The activity of the 40 kD chymotrypsin-like protease was found to be almost completely lost after insemination.

摘要

利用十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶对海胆卵提取物中的蛋白水解活性进行了检测。在未受精的卵中，在明胶凝胶上检测到蛋白酶呈现出对应分子量为40kD和26kD的条带，在酪蛋白凝胶上呈现出35kD和30kD的条带。使用各种蛋白酶抑制剂后发现，40kD、30kD和26kD的蛋白酶为类胰凝乳蛋白酶，35kD的蛋白酶为类胰蛋白酶。结果发现，受精后40kD类胰凝乳蛋白酶的活性几乎完全丧失。