海胆卵和胚胎中卵黄蛋白的降解

Degradation of yolk proteins in sea urchin eggs and embryos.

作者信息

Yokota Y, Kato K H

机构信息

Biological Laboratory, Aichi Prefectural University, Nagoya, Japan.

出版信息

Cell Differ. 1988 Apr;23(3):191-200. doi: 10.1016/0045-6039(88)90071-1.

DOI:10.1016/0045-6039(88)90071-1

PMID:3378269

Abstract

Yolk granules isolated from unfertilized and fertilized eggs of the sea urchins, Hemicentrotus pulcherrimus and Anthocidaris crassispina, were incubated in acidic media, and the protein components were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. By the incubation, a protein (molecular weight 180,000 in H. pulcherrimus and 178,000 in A. crassispina) most abundant in unfertilized eggs decreased, while proteins (molecular weight 61,000, 72,000, 94,000, 114,000 in H. pulcherrimus and 56,000, 70,000, 92,000, 112,000 in A. crassispina) dominant in developed embryos increased. Neither alkaline nor neutral condition resulted in such changes in the electrophoretic patterns of proteins as observed in acidic media. Experiments with various inhibitors of proteases suggested that thiol protease(s), such as cathepsin B, may be the most important enzyme(s) in the degradation of yolk proteins in embryogenesis of the sea urchin.

摘要

从海胆（光棘球海胆和厚刺海胆）未受精卵和受精卵中分离出的卵黄颗粒，在酸性培养基中孵育，然后通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分析蛋白质成分。通过孵育，未受精卵中含量最丰富的一种蛋白质（光棘球海胆中分子量为180,000，厚刺海胆中分子量为178,000）减少，而发育胚胎中占主导地位的蛋白质（光棘球海胆中分子量为61,000、72,000、94,000、114,000，厚刺海胆中分子量为56,000、70,000、92,000、112,000）增加。碱性和中性条件均未导致蛋白质电泳图谱出现如在酸性培养基中所观察到的变化。使用各种蛋白酶抑制剂进行的实验表明，巯基蛋白酶，如组织蛋白酶B，可能是海胆胚胎发育过程中卵黄蛋白降解的最重要酶类。