Mansfield Kylie J, Cerra Anna, Chamberlain Coral G
Department of Anatomy and Histology and Institute for Biomedical Research, The University of Sydney, New South Wales, Australia.
Mol Vis. 2004 Oct 6;10:728-37.
Many patients whose sight is initially restored by cataract surgery eventually suffer secondary loss of vision because of posterior capsule opacification (PCO; after-cataract), a condition in which lens epithelial cells left behind at surgery become aberrant and migrate into the light path. The aim of this study was to determine whether dexamethasone (DEX), an anti-inflammatory agent widely used before and after cataract surgery, influences the behavior of lens cells under conditions relevant to PCO development.
An established rat PCO model was used in which explanted epithelial cells attached to the lens capsule are exposed sequentially to TGFbeta2 and FGF-2. Cultures with or without DEX (100 nM), and appropriate controls, were maintained for up to 30 days and assessed by light and scanning electron microscopy or immunolocalization of PCO markers (alpha-smooth muscle actin or fibronectin) or a marker for lens epithelial cell phenotype (Pax-6).
In the absence of DEX, explants become multilayered and plaques that express PCO markers form. Cells tend to gather up into the plaques, leaving the surrounding lens capsule denuded. Changes in lens cell behavior with addition of DEX included rapid formation of long, needle-like cells, less extracellular matrix deposited on explant surface, and plaques surrounded by a monolayer of migratory cells. Immunolocalization confirmed that the latter were not normal lens epithelial cells.
Lens cell behavior in this PCO model was significantly affected by inclusion of DEX, highlighting the possibility that its use as an anti-inflammatory at the time of cataract surgery may influence PCO development.
许多最初通过白内障手术恢复视力的患者最终会因后囊膜混浊（PCO；后发性白内障）而继发视力丧失，在这种情况下，手术中残留的晶状体上皮细胞会发生异常并迁移到光路中。本研究的目的是确定地塞米松（DEX），一种在白内障手术前后广泛使用的抗炎剂，在与PCO发展相关的条件下是否会影响晶状体细胞的行为。
使用已建立的大鼠PCO模型，将附着在晶状体囊膜上的外植上皮细胞依次暴露于转化生长因子β2（TGFbeta2）和碱性成纤维细胞生长因子（FGF-2）。将含有或不含DEX（100 nM）的培养物以及适当的对照维持长达30天，并通过光学显微镜、扫描电子显微镜或PCO标志物（α-平滑肌肌动蛋白或纤连蛋白）或晶状体上皮细胞表型标志物（Pax-6）的免疫定位进行评估。
在没有DEX的情况下，外植体形成多层结构并形成表达PCO标志物的斑块。细胞倾向于聚集在斑块中，使周围的晶状体囊膜裸露。添加DEX后晶状体细胞行为的变化包括快速形成长的针状细胞，外植体表面沉积的细胞外基质减少，以及由单层迁移细胞包围的斑块。免疫定位证实后者不是正常的晶状体上皮细胞。
在该PCO模型中，DEX的加入显著影响了晶状体细胞的行为，突出了其在白内障手术时作为抗炎剂使用可能影响PCO发展的可能性。
