Zlobina E N, Dubinkin I V, Merkushov A V, Volynskaya N A, Gudima G O
Institute of Immunology, Moscow, U.S.S.R.
Immunol Lett. 1992 Feb 15;31(3):289-96. doi: 10.1016/0165-2478(92)90129-c.
The role of pancreatic beta-cell antigenic structures in modulation of insulin secretion in vitro was recently demonstrated by others. Here we report generation of a monoclonal antibody (mAB) ICA-1 to non-species specific beta-cell antigen(s) 64, 67 and 69 kDa. The mAB inhibits glucose stimulated insulin secretion in islet cell cultures. The ability of mAB ICA-1 to immunoprecipitate active glutamic acid decarboxylase from high speed supernatants of pancreatic and brain crude extracts was demonstrated. The 64, 67 and 69 kDa antigenic material was affinity purified from pancreatic islet cell high speed supernatants, active glutamic acid decarboxylase was found in the material. Immunoaffinity purification with mAB ICA-1 of GAD-like pancreatic beta-cell antigenic material has provided evidence of possible involvement of glutamic acid decarboxylase in modulation of insulin secretion.
近期其他人证实了胰腺β细胞抗原结构在体外调节胰岛素分泌中的作用。在此，我们报告了一种针对非物种特异性β细胞抗原（分子量为64、67和69 kDa）的单克隆抗体（mAB）ICA-1的产生。该单克隆抗体可抑制胰岛细胞培养物中葡萄糖刺激的胰岛素分泌。已证实mAB ICA-1能够从胰腺和脑粗提物的高速上清液中免疫沉淀活性谷氨酸脱羧酶。从胰岛细胞高速上清液中亲和纯化出了分子量为64、67和69 kDa的抗原物质，该物质中发现了活性谷氨酸脱羧酶。用mAB ICA-1对胰腺β细胞类谷氨酸脱羧酶抗原物质进行免疫亲和纯化，为谷氨酸脱羧酶可能参与胰岛素分泌调节提供了证据。
