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腺病毒载体经肝动脉隔离灌注后中和抗体形成受损及转导效率高。

Impaired neutralising antibody formation and high transduction efficacy after isolated hepatic perfusion with adenoviral vectors.

作者信息

van Etten B, Eggermont A M M, Ambagtsheer G, van Tiel S T, ten Hagen T L M

机构信息

Erasmus University Medical Centre-Daniel den Hoed Cancer Centre, Department of Surgical Oncology, PO Box 5201, 3008 AE Rotterdam, The Netherlands.

出版信息

Br J Cancer. 2004 Oct 18;91(8):1610-3. doi: 10.1038/sj.bjc.6602151.

DOI:10.1038/sj.bjc.6602151
PMID:15480435
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2409916/
Abstract

Local adenoviral gene transfer can be performed by means of isolated hepatic perfusion (IHP). This methodology is a very effective and safe way to deliver adenoviral vectors. We studied the immune response after IHP. A decreased neutralising antibody formation was observed, offering possibilities for further research in the field of gene therapy in isolated perfusion settings.

摘要

局部腺病毒基因转移可通过离体肝脏灌注（IHP）来进行。这种方法是递送腺病毒载体的一种非常有效且安全的方式。我们研究了离体肝脏灌注后的免疫反应。观察到中和抗体形成减少，这为在离体灌注环境下的基因治疗领域进一步研究提供了可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f038/2409916/142ce6beb58c/91-6602151f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f038/2409916/4039038e5836/91-6602151f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f038/2409916/142ce6beb58c/91-6602151f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f038/2409916/4039038e5836/91-6602151f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f038/2409916/142ce6beb58c/91-6602151f2.jpg

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Impaired neutralising antibody formation and high transduction efficacy after isolated hepatic perfusion with adenoviral vectors.腺病毒载体经肝动脉隔离灌注后中和抗体形成受损及转导效率高。
Br J Cancer. 2004 Oct 18;91(8):1610-3. doi: 10.1038/sj.bjc.6602151.
2
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本文引用的文献

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Prerequisites for effective adenovirus mediated gene therapy of colorectal liver metastases in the rat using an intracellular neutralizing antibody fragment to p21-Ras.使用针对p21-Ras的细胞内中和抗体片段对大鼠结直肠癌肝转移进行有效腺病毒介导基因治疗的前提条件。
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Neighborhood politics: the immunoregulatory function of organ-resident liver endothelial cells.邻里政治：器官驻留肝内皮细胞的免疫调节功能
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An immunomodulatory procedure that stabilizes transgene expression and permits readministration of E1-deleted adenovirus vectors.
一种免疫调节程序，可稳定转基因表达并允许再次施用E1缺失的腺病毒载体。
Mol Ther. 2001 Mar;3(3):293-301. doi: 10.1006/mthe.2000.0258.
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Adenovirus-mediated interleukin 3 beta gene transfer by isolated limb perfusion inhibits growth of limb sarcoma in rats.通过离体肢体灌注进行腺病毒介导的白细胞介素3β基因转移可抑制大鼠肢体肉瘤的生长。
Hum Gene Ther. 2001 Mar 20;12(5):489-502. doi: 10.1089/104303401300042384.
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Factors influencing in vivo transduction by recombinant adeno-associated viral vectors expressing the human factor IX cDNA.影响表达人凝血因子IX cDNA的重组腺相关病毒载体体内转导的因素。
Blood. 2001 Mar 1;97(5):1258-65. doi: 10.1182/blood.v97.5.1258.
6
Efficient presentation of exogenous antigen by liver endothelial cells to CD8+ T cells results in antigen-specific T-cell tolerance.肝内皮细胞向外源性抗原向CD8 + T细胞的有效呈递导致抗原特异性T细胞耐受。
Nat Med. 2000 Dec;6(12):1348-54. doi: 10.1038/82161.
7
Isolated limb perfusion for local gene delivery: efficient and targeted adenovirus-mediated gene transfer into soft tissue sarcomas.用于局部基因递送的离体肢体灌注：高效且靶向的腺病毒介导的基因转移至软组织肉瘤
Ann Surg. 2000 Dec;232(6):814-21. doi: 10.1097/00000658-200012000-00010.
8
Tumor necrosis factor-alpha augments tumor effects in isolated hepatic perfusion with melphalan in a rat sarcoma model.在大鼠肉瘤模型中，肿瘤坏死因子-α增强了美法仑隔离肝灌注的肿瘤效应。
J Immunother. 2000 Jul-Aug;23(4):449-55. doi: 10.1097/00002371-200007000-00008.
9
Immunological tolerance--the liver effect.免疫耐受——肝脏效应。
Immunol Rev. 2000 Apr;174:280-2. doi: 10.1034/j.1600-0528.2002.017419.x.
10
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J Clin Microbiol. 1999 Dec;37(12):3940-5. doi: 10.1128/JCM.37.12.3940-3945.1999.