Wang Xinglong, Liu Sixiu, Liu Xiaojun, Chen Zhonghai, Liu Xuefen, Pang Yongzhen, Sun Xiaofen, Tang Kexuan
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Morgan-Tan International Center for Life Sciences, Fudan University, Shanghai 200433, P.R. China.
DNA Seq. 2004 Jun;15(3):180-7. doi: 10.1080/10425170410001704508.
A new CBF gene was cloned from Capsella bursa-pastoris(shepherd's purse) by rapid amplification of cDNA ends (RACE). The full-length cDNA of C. bursa-pastoris CBF gene (designated as Cbcbf) was 1034 bp long and contained a 657 bp open reading frame (ORF) encoding a putative DRE/CRT (LTRE)-binding protein of 219 amino acids. The predicted CbCBF protein was found to have a potential nuclear localization signal (NLS) in its N-terminal region followed by an AP2 DNA-binding motif and an acidic C-terminal half that might act as an activator domain. Bioinformatic analysis revealed that Cbcbf strongly resembled other CBF genes from Arabidopsis thaliana (cbf1, cbf2, cbf3) and Brassica napus (Bncbf5, Bncbf 7, Bncbf16 and Bncbf17). Subsequent cold acclimation assay showed that Cbcbf was relevant to cold acclimation. Our study implies that Cbcbf might have similar functions possessed by other CBF genes such as inducing the expression of some cold-regulated genes and increasing plants' freezing tolerance.
通过cDNA末端快速扩增（RACE）技术，从荠菜中克隆出一个新的CBF基因。荠菜CBF基因（命名为Cbcbf）的全长cDNA为1034 bp，包含一个657 bp的开放阅读框（ORF），编码一个由219个氨基酸组成的假定的DRE/CRT（LTRE）结合蛋白。预测的CbCBF蛋白在其N端区域有一个潜在的核定位信号（NLS），随后是一个AP2 DNA结合基序和一个可能作为激活域的酸性C端半区。生物信息学分析表明，Cbcbf与拟南芥（cbf1、cbf2、cbf3）和甘蓝型油菜（Bncbf5、Bncbf 7、Bncbf16和Bncbf17）的其他CBF基因高度相似。随后的冷驯化试验表明，Cbcbf与冷驯化有关。我们的研究表明，Cbcbf可能具有其他CBF基因所具有的类似功能，如诱导一些冷调节基因的表达和提高植物的抗冻性。
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