Lin Juan, Zhang Wen, Shi Mingzhu, Wang Xinglong, Sun Xiaofen, Tang Kexuan
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai, People's Republic of China.
Biocell. 2008 Dec;32(3):229-35.
A new cation exchangers (CAXs) gene was cloned and characterized from Capsella bursa-pastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence of cax from C. bursa-pastoris (designated as Cbcax51) was 1754 bp containing a 1398 bp open reading frame encoding a polypeptide of 466 amino-acid residues with a calculated molecular mass of 50.5 kDa and an isoelectric point of 5.69. The predicted CbCAX51 contained an IMP dehydrogenase/GMP reductase domain, two Na+/Ca2+ exchanger protein domains. Comparative and bioinformatics analyses revealed that CbCAX51 showed extensive homology with CAX from other plant species. The expression analysis by different treatments indicated that Cbcax51 could be activated by cold triggering and was related to the cold acclimation process, but its expression is regulated negatively by drought and not affected by ABA or salt.
通过cDNA末端快速扩增（RACE）技术，从荠菜中克隆并鉴定了一个新的阳离子交换体（CAXs）基因。荠菜cax的全长cDNA序列（命名为Cbcax51）为1754 bp，包含一个1398 bp的开放阅读框，编码一个由466个氨基酸残基组成的多肽，计算分子量为50.5 kDa，等电点为5.69。预测的CbCAX51包含一个肌苷酸脱氢酶/鸟苷酸还原酶结构域和两个Na+/Ca2+交换蛋白结构域。比较分析和生物信息学分析表明，CbCAX51与其他植物物种的CAX具有广泛的同源性。不同处理的表达分析表明，Cbcax51可被冷诱导激活，与冷驯化过程有关，但其表达受到干旱的负调控，不受脱落酸或盐的影响。
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