Sheng Yanjuan, Zhu Yu, Wu Lezheng
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Yan Ke Xue Bao. 2004 Sep;20(3):181-6.
To investigate the effect of high dose methylprednisolone (MP) on retinal ganglion cells (RGC) apoptosis.
One hundred and twenty six Wistar rats were divided into normal control, crush control and MP treatment groups. High dose MP and 0.9% sodium chloride were injected intravenously at 1 h after injury in the treatment group and crush control group, respectively. Apoptotic cells, Bcl-2 and Bax positive cells were located and calculated by terminal-deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling (TUNEL), immunohistochemical method and retinal whole-mount combined with section technique at day 4, 7, 14 after crush, respectively.
The TUNEL-labeling cells, Bcl-2 and Bax positive cells were detected in the ganglion cell layer of retina. Few apoptotic cells, a few of Bcl-2 and Bax positive cells were observed in normal control groups. The numbers of positive cells increased gradually after crush. The number of apoptotic cells and Bax positive tells in treatment groups was less than that in crush group significantly (P < 0.05), while the number of Bcl-2 positive cells in treatment group was more than that in crush group at day 4, 7 and 14 after crush significantly (P < 0.05).
These results suggest that high dose MP can inhibit the apoptosis of RGC after optic nerve crush probably through up-regulation of Bcl-2 expression and down-regulation of Bax expression.
研究大剂量甲基强的松龙（MP）对视网膜神经节细胞（RGC）凋亡的影响。
将126只Wistar大鼠分为正常对照组、挤压对照组和MP治疗组。治疗组和挤压对照组分别在损伤后1小时静脉注射大剂量MP和0.9%氯化钠。分别在挤压后第4、7、14天，通过末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记法（TUNEL）、免疫组织化学方法以及视网膜铺片联合切片技术定位并计算凋亡细胞、Bcl-2和Bax阳性细胞。
在视网膜神经节细胞层检测到TUNEL标记细胞、Bcl-2和Bax阳性细胞。正常对照组观察到很少的凋亡细胞、少量的Bcl-2和Bax阳性细胞。挤压后阳性细胞数量逐渐增加。治疗组凋亡细胞和Bax阳性细胞数量明显少于挤压组（P<0.05），而在挤压后第4、7、14天，治疗组Bcl-2阳性细胞数量明显多于挤压组（P<0.05）。
这些结果表明，大剂量MP可能通过上调Bcl-2表达和下调Bax表达来抑制视神经挤压后RGC的凋亡。
