Site-specific PEGylation of proteins containing unnatural amino acids.
作者信息
Deiters Alexander, Cropp T Ashton, Summerer Daniel, Mukherji Mridul, Schultz Peter G
机构信息
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
出版信息
Bioorg Med Chem Lett. 2004 Dec 6;14(23):5743-5. doi: 10.1016/j.bmcl.2004.09.059.
PMID:15501033
Abstract
Here, we report a generally applicable PEGylation methodology based on the site-specific incorporation of para-azidophenylalanine into proteins in yeast. The azido group was used in a mild [3+2] cycloaddition reaction with an alkyne derivatized PEG reagent to afford selectively PEGylated protein. This strategy should be useful for the generation of selectively PEGylated proteins for therapeutic applications.
摘要
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