Huang Y-C, Su L-H, Wu T-L, Lin T-Y
Division of Paediatric Infectious Diseases, Chang Gung Children's Hospital, Chang Gung University, 5, Fu-Shin Street, Kweishan, Taoyuan, Taiwan.
J Hosp Infect. 2004 Nov;58(3):200-3. doi: 10.1016/j.jhin.2004.07.020.
To analyse the relatedness of colonizing candidal isolates from very-low-birthweight infants hospitalized in a neonatal intensive care unit (NICU), we prospectively collected 86 candidal isolates from 20 infants, including 67 isolates of Candida albicans from 15 infants, 17 isolates of Candida parapsilosis from five infants and two isolates of Candida glabrata from one infant, who also had C. albicans colonization, over a one-year period. All 86 isolates were genotyped by infrequent-restriction-site polymerase chain reaction (IRS-PCR) and electrophoretic karyotyping (EK) with pulsed-field gel electrophoresis. A total of 15 genotypes were identified by IRS-PCR and 12 genotypes by EK. Some infants shared a common genotype. From a single infant, an identical genotype was found in 11 of 13 cases where at least two isolates of same Candida species were available for genotyping analysis, regardless of anatomical site, how many isolates were recovered or how many times. Should an infant harbour a candidal strain, they may harbour this strain at multiple sites and for a prolonged period.
为分析在新生儿重症监护病房（NICU）住院的极低出生体重儿中定植念珠菌分离株的相关性，我们在一年时间里前瞻性地收集了来自20名婴儿的86株念珠菌分离株，其中包括来自15名婴儿的67株白色念珠菌分离株、来自5名婴儿的17株近平滑念珠菌分离株以及来自1名同时伴有白色念珠菌定植的婴儿的2株光滑念珠菌分离株。所有86株分离株均通过低频限制性位点聚合酶链反应（IRS-PCR）以及采用脉冲场凝胶电泳的电泳核型分析（EK）进行基因分型。通过IRS-PCR共鉴定出15种基因型，通过EK鉴定出12种基因型。部分婴儿具有相同的基因型。在同一婴儿中，在13例至少有两株相同念珠菌属分离株可用于基因分型分析的病例中，有11例发现了相同的基因型，无论解剖部位、回收的分离株数量或次数如何。如果婴儿携带一种念珠菌菌株，他们可能会在多个部位长期携带该菌株。
