Vrioni G, Matsiota-Bernard P
Laboratoire de Microbiologie, Hôpital Raymond Poincaré, 92380 Garches, France.
J Infect. 2001 Jan;42(1):50-6. doi: 10.1053/jinf.2000.0778.
The aim of our study was the molecular typing of 40 clinical isolates of Candida spp. obtained from patients with burns or trauma hospitalized in the intensive care unit of a general hospital.
Isolates were recovered from blood, deep trauma, urine, sputum or from environment within a short period of time (4 months). The yeasts were identified using commercial yeast identification kits as C. albicans (17 isolates), C. tropicalis (16 isolates) and C. parapsilosis (10 isolates). The epidemiological relation of the isolates was tested with the Random Amplified Polymorphic DNA assay using three or four arbitrary chosen primers.
All C. albicans isolates presented distinct RAPD profiles, C. tropicalis isolates presented both the same and distinct RAPD patterns and the C. parapsilosis isolates presented the same RAPD pattern. All the environmental isolates were identified as C. parapsilosis and they had the same RAPD pattern as C. parapsilosis clinical isolates. Candida parapsilosis delineation was confirmed with PFGE.
The colonization/infection with C. albicans was endogenous, the C. tropicalis colonization/infection was both endogenous and exogenous, and the C. parapsilosis colonization/infection had an environmental origin.
我们研究的目的是对从一家综合医院重症监护病房住院的烧伤或创伤患者中分离出的40株念珠菌属临床菌株进行分子分型。
在短时间内（4个月）从血液、深部创伤、尿液、痰液或环境中分离出菌株。使用商业酵母鉴定试剂盒将酵母鉴定为白色念珠菌（17株）、热带念珠菌（16株）和近平滑念珠菌（10株）。使用三到四个任意选择的引物，通过随机扩增多态性DNA分析来检测分离株之间的流行病学关系。
所有白色念珠菌分离株呈现出不同的RAPD图谱，热带念珠菌分离株呈现出相同和不同的RAPD模式，而近平滑念珠菌分离株呈现出相同的RAPD模式。所有环境分离株均被鉴定为近平滑念珠菌，并且它们与近平滑念珠菌临床分离株具有相同的RAPD模式。通过脉冲场凝胶电泳（PFGE）证实了近平滑念珠菌的分型。
白色念珠菌的定植/感染是内源性的，热带念珠菌的定植/感染既有内源性又有外源性，而近平滑念珠菌的定植/感染起源于环境。
