Davies S, Richardson M C, Anthony F W, Mukhtar D, Cameron I T
Maternal, Fetal and Neonatal Physiology, Developmental Origins of Health and Disease Division, University of Southampton, Level F (815), Princess Anne Hospital, Coxford Road, Southampton SO16 5YA, UK.
Mol Hum Reprod. 2004 Dec;10(12):935-9. doi: 10.1093/molehr/gah124. Epub 2004 Oct 22.
The Fallopian tube provides the environment for early embryo growth, a process which is influenced by insulin-like growth factors (IGFs) in the tubal fluid. Whether the bioavailability of tubal IGFs is modulated by locally produced IGF-binding protein (IGFBP-1) is not clear. An explant culture system from human Fallopian tube mucosa was, therefore, developed enabling the potential for IGFBP-1 production by this tissue to be examined directly. Initial characterization of the system established that the explants maintained responsiveness to steroids. Thus, oviduct-specific glycoprotein production, a major product of the oviduct in vivo, continued to be made via an estrogen-sensitive pathway in the culture. The presence of mRNA for IGFBP-1 was established within the explants by the use of quantitative RT-PCR and IGFBP-1 protein was measured by enzyme-linked immunosorbent assay. Although insulin and estradiol had no consistent effect on IGFBP-1, addition of progesterone had a significant inhibitory effect on IGFBP-1 production, both at the mRNA and protein levels. A dose-range of progesterone revealed an incremental inhibitory effect of progesterone on IGFBP-1 output (maximal effect, 25-50 nmol/l), consistent with physiological inhibition of this process during the luteal phase. We suggest that progesterone might, therefore, play a role in controlling the bioavailability of IGFs to the embryo during early development within the Fallopian tube.
输卵管为早期胚胎生长提供环境，这一过程受输卵管液中胰岛素样生长因子（IGFs）的影响。输卵管IGFs的生物利用度是否受局部产生的IGF结合蛋白（IGFBP - 1）调节尚不清楚。因此，开发了一种来自人输卵管黏膜的外植体培养系统，能够直接检测该组织产生IGFBP - 1的潜力。该系统的初步特性表明外植体对类固醇保持反应性。因此，输卵管特异性糖蛋白的产生，这是输卵管在体内的主要产物，在培养中通过雌激素敏感途径持续产生。通过定量RT - PCR确定外植体内存在IGFBP - 1的mRNA，并通过酶联免疫吸附测定法测量IGFBP - 1蛋白。虽然胰岛素和雌二醇对IGFBP - 1没有一致的影响，但添加孕酮对IGFBP - 1的产生在mRNA和蛋白水平均有显著抑制作用。孕酮的剂量范围显示其对IGFBP - 1输出有递增的抑制作用（最大效应，25 - 50 nmol/l），这与黄体期该过程的生理抑制一致。因此，我们认为孕酮可能在输卵管内早期发育过程中控制IGFs对胚胎的生物利用度方面发挥作用。
