骨骼肌肌球蛋白导致脑微管解聚

Depolymerization of brain microtubules by skeletal muscle myosin.

作者信息

Hayashi M

出版信息

J Biochem. 1979 Mar;85(3):691-8.

Abstract

Brain microtubules are found to disperse rods of skeletal muscle myosin and become decorated with amorphous aggregates of myosin. Then microtubules are partially depolymerized by myosin. Myosin shows high Mg2+-GTPase activity which is not influenced by microtubules, and induces the partial depolymerization of microtubules by exhaustion of GTP in the solution. H-meromyosin depolymerizes microtubules like myosin does. H-meromyosin is, however, contaminated with a trace amount of trypsin, which irreversibly depolymerizes microtubules.

摘要

发现脑微管会分散骨骼肌肌球蛋白的棒状物，并被肌球蛋白的无定形聚集体所修饰。然后微管会被肌球蛋白部分解聚。肌球蛋白表现出高Mg2+ - GTP酶活性，该活性不受微管影响，并通过耗尽溶液中的GTP诱导微管部分解聚。重酶解肌球蛋白像肌球蛋白一样使微管解聚。然而，重酶解肌球蛋白被痕量的胰蛋白酶污染，胰蛋白酶会不可逆地使微管解聚。