Goseki N, Yamazaki S, Endo M, Onodera T, Kosaki G, Hibino Y, Kuwahata T
First Department of Surgery, Tokyo Medical and Dental University School of Medicine, Japan.
Cancer. 1992 Apr 1;69(7):1865-72. doi: 10.1002/1097-0142(19920401)69:7<1865::aid-cncr2820690732>3.0.co;2-m.
Methionine-depleting total parenteral nutrition (methionine-depleting TPN), which infuses an amino acid solution devoid of L-methionine and L-cysteine as the sole protein source, showed enhancement of the effect of several anti-cancer agents. In this study, the combined effect of the methionine-depleting TPN with the administration of doxorubicin was examined in Yoshida sarcoma (YS)-bearing rats with regard to effects on the primary tumor growth, the extension of metastasis, and the host animal's life span. In the first experiment, immediately after receiving methionine-depleting TPN for 8 days, the animals were killed. Pathologic findings evaluated tumor growth in the implanted site and extension of the metastasis. In the second experiment, the survival period was determined after animals received methionine-depleting TPN for 10 days, with subsequent oral feeding until they died naturally. Proliferation of YS was markedly suppressed. In particular, hematogenous metastasis, which is a characteristic of YS, was suppressed, and a longer survival period (42.7 +/- 15.6 days, mean +/- SD) was attained in rats in the group treated with the methionine-depleting TPN combined with the administration of doxorubicin.
蛋氨酸消耗型全胃肠外营养（蛋氨酸消耗型TPN），即输注不含L-蛋氨酸和L-半胱氨酸的氨基酸溶液作为唯一蛋白质来源，显示出增强了几种抗癌药物的效果。在本研究中，针对对原发性肿瘤生长、转移扩展和宿主动物寿命的影响，在荷吉田肉瘤（YS）大鼠中检测了蛋氨酸消耗型TPN与阿霉素给药的联合效果。在第一个实验中，动物接受蛋氨酸消耗型TPN 8天后立即处死。病理检查结果评估了植入部位的肿瘤生长和转移扩展情况。在第二个实验中，动物接受蛋氨酸消耗型TPN 10天，随后经口喂养直至自然死亡，测定其生存期。YS的增殖明显受到抑制。特别是，作为YS特征的血行转移受到抑制，在接受蛋氨酸消耗型TPN联合阿霉素给药治疗的大鼠组中，生存期延长（42.7±15.6天，平均值±标准差）。
