Wozniak Waldemar, Markuszewski Jacek, Wierusz-Kozlowska Malgorzata, Wysocki Henryk
Department of Orthopedics, Poznan University of Medical Sciences, Poland.
Acta Orthop Scand. 2004 Oct;75(5):549-53. doi: 10.1080/00016470410001402.
Polymorphonuclear neutrophils (PMN) are the first cells to take part in the local foreign body reaction in aseptic loosening of endoprostheses. The aim of this study was to evaluate the systemic host reaction to total joint replacement by measuring the production of nitric oxide by neutrophils before and after total joint replacement.
Blood samples were collected from 33 patients (27 hips and 6 knees) before surgery, and 2 weeks, 2 months and 2.5-3 years after surgery. The levels of nitric oxide produced by PMN were measured by the method described by Markert et al. (1994).
Patients reporting pain in the region of the implant 3 years after surgery, and also patients with radiographic signs of loosening, had higher production of NO in the early period and 3 years after the implantation than those with good clinical results.
We propose that elevated levels of nitric oxide production by PMNs may serve as a marker of total joint prosthesis loosening.
多形核中性粒细胞（PMN）是参与假体无菌性松动局部异物反应的首批细胞。本研究的目的是通过测量全关节置换术前和术后中性粒细胞产生一氧化氮的情况，评估全身宿主对全关节置换的反应。
采集33例患者（27例髋关节和6例膝关节）术前、术后2周、2个月及2.5 - 3年的血样。PMN产生的一氧化氮水平采用Markert等人（1994年）描述的方法进行测量。
术后3年报告植入部位疼痛的患者，以及有影像学松动迹象的患者，在植入早期和3年后的一氧化氮产生量高于临床效果良好的患者。
我们认为PMN产生一氧化氮水平升高可能作为全关节假体松动的一个标志物。
