构成分离中期染色体外表面的蛋白质的定位
Localization of proteins forming the outer surface of isolated metaphase chromosomes.
作者信息
Belyaev N D, Budker V G, Dubrovskaya V A, Kim A A, Kiseleva E V, Sidorov V N
机构信息
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences.
出版信息
FEBS Lett. 1992 Feb 3;297(1-2):43-5. doi: 10.1016/0014-5793(92)80323-9.
The outer surface of isolated metaphase chromosomes has been investigated by a method of thermally activated tritium labelling. We show that both chromosomal proteins and DNA are tritium-labelled. Fractionation of the chromosomal proteins reveals that scaffold proteins are the most labelled in condensed and EDTA-decondensed chromosomes. Exposition of some scaffold proteins on the outer surface of metaphase chromosomes is suggested.
采用热激活氚标记法对分离出的中期染色体的外表面进行了研究。我们发现染色体蛋白质和DNA都被氚标记了。对染色体蛋白质进行分级分离后发现，在浓缩和经乙二胺四乙酸（EDTA）解聚的染色体中，支架蛋白的标记程度最高。这表明中期染色体外表面存在一些支架蛋白。
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