Mace M L, Daskal Y, Busch H, Wray V P, Wray W
Cytobios. 1977;19(73):27-40.
Scanning electron microscopy of salt extracted, isolated metaphase chromosomes has been used to further the understanding of the role of proteins and DNA in chromosome architecture. Metaphase chromosome surfaces consist of numerous compact microconvules with an average diameter of 520 +/- 78 A which result from several orders of supercoiling. Treatment of isolated chromosomes with various salt concentrations ranging from 0.4 to 2.0 M NaCl revealed a longitudinal array of fibres within the chromatids as well as regions of coalesced nucleoprotein. The results demonstrate sensitivity of chromosome structure based on chromatin supercoiling, to progressive extraction of chromosomal proteins and DNA over the range of salt concentrations examined.
利用扫描电子显微镜对盐提取的分离中期染色体进行观察，以进一步了解蛋白质和DNA在染色体结构中的作用。中期染色体表面由许多紧密的微卷曲组成，平均直径为520±78埃，这是由几个层次的超螺旋形成的。用0.4至2.0M NaCl的各种盐浓度处理分离的染色体，结果显示染色单体中有纵向排列的纤维以及凝聚的核蛋白区域。结果表明，在所检测的盐浓度范围内，基于染色质超螺旋的染色体结构对染色体蛋白质和DNA的逐步提取具有敏感性。
