Adam Ahmed Abdel Gadir, Takahashi Yoshiyuki, Katagiri Seiji, Nagano Masashi
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
J Reprod Dev. 2004 Oct;50(5):579-86. doi: 10.1262/jrd.50.579.
To develop a reliable follicle culture system, mouse preantral follicles 150-200 microm in diameter were cultured individually for 5 or 6 days in membrane inserts or in droplets, and then induced to ovulate with hCG (Experiment 1). The nuclear maturation and developmental competence of the oocytes that ovulated from the follicles cultured in inserts were determined (Experiment 2). There was no significant difference between the two culture systems in the survival rate (83 and 77%). However, follicles cultured in inserts showed a higher ovulation rate (63%) than those cultured in droplets (39%, P<0.05). About 80% of the oocytes that ovulated from the follicles cultured in inserts were at the metaphase II stage. After in vitro fertilization, 75 and 48% of in vitro ovulated oocytes cleaved and developed into blastocysts, respectively. These results demonstrate that the insert culture system is superior to the droplet culture system in terms of follicular growth and ovulation, and can be used to investigate the growth and ovulation of follicles in vitro.
为建立可靠的卵泡培养系统，将直径为150 - 200微米的小鼠窦前卵泡分别在膜插入物或液滴中培养5或6天，然后用hCG诱导排卵（实验1）。测定从在插入物中培养的卵泡排出的卵母细胞的核成熟和发育能力（实验2）。两种培养系统在存活率方面无显著差异（分别为83%和77%）。然而，在插入物中培养的卵泡排卵率（63%）高于在液滴中培养的卵泡（39%，P<0.05）。从在插入物中培养的卵泡排出的卵母细胞约80%处于中期II阶段。体外受精后，体外排卵的卵母细胞分别有75%和48%发生卵裂并发育成囊胚。这些结果表明，在卵泡生长和排卵方面，插入物培养系统优于液滴培养系统，可用于体外研究卵泡的生长和排卵。
