Urathamakul Thitima, Beck Jennifer L, Sheil Margaret M, Aldrich-Wright Janice R, Ralph Stephen F
Department of Chemistry, University of Wollongong, Northfields Avenue, New South Wales, Australia.
Dalton Trans. 2004 Sep 7(17):2683-90. doi: 10.1039/B406889K. Epub 2004 Jul 29.
Electrospray ionisation mass spectrometry was used to investigate reactions between six ruthenium compounds and three different non self-complementary duplex oligonucleotides containing 16 base pairs. Each of the compounds studied formed non-covalent complexes containing between one and five ruthenium molecules bound to DNA. Competition experiments involving duplex 16mers and pairs of ruthenium compounds were used to determine the order of relative binding affinities of the metal compounds. Other competition experiments involving ruthenium compounds, and the organic DNA binding agents daunomycin and distamycin, provided information about the sites and modes of DNA binding of the ruthenium compounds.
采用电喷雾电离质谱法研究了六种钌化合物与三种不同的含16个碱基对的非自互补双链寡核苷酸之间的反应。所研究的每种化合物都形成了非共价复合物，其中含有一至五个与DNA结合的钌分子。涉及双链16聚体和钌化合物对的竞争实验用于确定金属化合物相对结合亲和力的顺序。其他涉及钌化合物以及有机DNA结合剂柔红霉素和distamycin的竞争实验提供了有关钌化合物DNA结合位点和模式的信息。
