Qin Xing-jun, Qiu Pan, Wang Xu-kai, Sun Chang-fu, Li Qing-chang, Han Yu-chen, Guan Xiao-feng
Department of Oral Maxillofacial Surgery, The First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Shanghai Kou Qiang Yi Xue. 2004 Oct;13(5):421-5.
To investigate the expression and function of the adhesion molecules ICAM-3, CD34 and HLA-DR antigen on endothelial cells of hemangiomas in different stages.
SABC immunohistochemical technique was used to examine the expression of ICAM-3, CD34 and HLA-DR on vascular endothelial cells. Seventy-six specimens including 28 proliferating hemangiomas, 22 involuting hemangiomas, 18 vascular deformity and 8 normal skin tissue were obtained from infants and children for the experiment.
The results showed: (1) both ICAM-3 and CD34 had high expression in proliferating hemangiomas, but poor or even no expression in involuting hemangiomas. There was a significant difference (P<0.001) between the two phases. Both ICAM-3 and CD34 had almost no expression in vascular deformity and normal skin tissue, significantly different from hemangiomas (P<0.001).(2) HLA-DR expression was closely related to the high differentiation phase of vascular epithelial cells. In the proliferating phase, HLA-DR didn't express, while it expressed highly as endothelial cells were in well-matured involuting phase. These differences were significant (P<0.001).
ICAM-3 and CD34 might play a role in the early stage of angiogenesis and take part in the pathological genesis and regression process of hemangiomas by regulating endothelial cells adhesion; HLA-DR might be related either to acquisition of a mature phenotype or to an activated state of the endothelial cells.
研究不同阶段血管瘤内皮细胞上黏附分子ICAM - 3、CD34和HLA - DR抗原的表达及功能。
采用SABC免疫组化技术检测ICAM - 3、CD34和HLA - DR在血管内皮细胞上的表达。从婴幼儿获取76份标本，包括28例增殖期血管瘤、22例消退期血管瘤、18例血管畸形和8例正常皮肤组织用于实验。
结果显示：（1）ICAM - 3和CD34在增殖期血管瘤中均高表达，但在消退期血管瘤中表达较弱甚至无表达。两个阶段之间差异有统计学意义（P<0.001）。ICAM - 3和CD34在血管畸形和正常皮肤组织中几乎无表达，与血管瘤差异显著（P<0.001）。（2）HLA - DR表达与血管内皮细胞的高分化阶段密切相关。在增殖期，HLA - DR不表达，而在内皮细胞处于成熟良好的消退期时高表达。这些差异有统计学意义（P<0.001）。
ICAM - 3和CD34可能在血管生成早期起作用，并通过调节内皮细胞黏附参与血管瘤的病理发生和消退过程；HLA - DR可能与内皮细胞成熟表型的获得或激活状态有关。
