Komatsu Teruyuki, Yamamoto Hisashi, Huang Yubin, Horinouchi Hirohisa, Kobayashi Koichi, Tsuchida Eishun
Advanced Research Institute for Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
J Biomed Mater Res A. 2004 Dec 15;71(4):644-51. doi: 10.1002/jbm.a.30200.
Recombinant human serum albumin (rHSA) incorporating the synthetic heme "albumin-heme" is an oxygen-carrying plasma protein that has the potential to be a red blood cell substitute. The physiological responses to a 30% exchange transfusion with two types of albumin-heme (rHSA-FecycP, rHSA-FepivP) solutions after 70% isovolemic hemodilution with 5 g/dL rHSA were investigated using anesthetized rats. The circulation parameters, blood parameters, renal cortical oxygen pressure (pO2), and muscle tissue pO2 were carefully monitored for 60 min after the injection. The declined mean arterial pressure and the mixed venous partial pO2 significantly recovered to 70.8 and 91.9% of the basal values by intravenous infusion of albumin-hemes, respectively. The lowered renal cortical pO2 also increased, indicating oxygen transport by this synthetic hemoprotein. The administration of albumin-heme into the acute anemia rat model after hemorrhage improved the circulatory volume and resuscitated the shock state. Both rHSA-FecycP and rHSA-FepivP transported oxygen through the body.
包含合成血红素“白蛋白 - 血红素”的重组人血清白蛋白（rHSA）是一种携氧血浆蛋白，有潜力成为红细胞替代品。使用麻醉大鼠研究了在5 g/dL rHSA进行70%等容血液稀释后，用两种白蛋白 - 血红素（rHSA - FecycP、rHSA - FepivP）溶液进行30%换血输血后的生理反应。注射后60分钟内仔细监测循环参数、血液参数、肾皮质氧分压（pO2）和肌肉组织pO2。通过静脉输注白蛋白 - 血红素，下降的平均动脉压和混合静脉血氧分压分别显著恢复到基础值的70.8%和91.9%。降低的肾皮质pO2也升高，表明这种合成血红蛋白可运输氧气。在出血后的急性贫血大鼠模型中给予白蛋白 - 血红素可改善循环血量并复苏休克状态。rHSA - FecycP和rHSA - FepivP均可在体内运输氧气。
