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Correlation between insertion mutant activities and amino acid sequence identities of the TaqI and TthHB8 restriction endonucleases.

作者信息

Barany F, Zebala J

机构信息

Department of Microbiology, Hearst Microbiology Research Center, Cornell University Medical College, New York, NY 10021.

出版信息

Gene. 1992 Mar 1;112(1):13-20. doi: 10.1016/0378-1119(92)90297-3.

DOI:10.1016/0378-1119(92)90297-3
PMID:1551592
Abstract

A two-codon insertion mutagenesis method has been generalized. Over two dozen insertion mutants throughout the gene encoding TaqI restriction endonuclease were constructed and activity was characterized. All mutants with activity either cleaved or nicked the canonical T decreases CGA recognition sequence. Some insertion mutants created duplication of gene regions, termed Gemini proteins, which still retained activity. The correlation between mutants with poor activity and the regions of shared amino acid identity between the isoschizomeric TaqI and TthHB8I suggests these regions are involved in DNA recognition and/or catalysis.

摘要

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