Lanza Henrique, Afonso-Cardoso Sandra R, Silva Adriano G, Napolitano Danielle R, Espíndola Foued S, Pena Janethe D O, Souza Maria A
Laboratório de Imunologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil.
Biol Res. 2004;37(3):385-93. doi: 10.4067/s0716-97602004000300004.
Amastigotes of Leishmania major have a great ability to evade destruction in host cells. This study investigated the activation in resident, inflammatory macrophages and J774 cells in vitro treated with lipopolysaccharide (LPS), soluble Leishmania antigen (SLA), calcium ionophore (CaI) and magnesium (Mg2+) alone or combined. An increase in nitric oxide (NO) production was observed in J774 or inflammatory macrophages treated with LPS alone or in combination with SLA and CaI. The same treatments did not affect the NO release by resident macrophages. There was no interference in uptake of L. major but CaI decreased intracellular proliferation of the parasite. This study demonstrated the importance of CaI in decreasing L. major proliferation inside murine macrophages while Mg2+ seemed to increase parasite proliferation. These finding may help to understand the events involved in host cells' clearance of this pathogen.
硕大利什曼原虫的无鞭毛体在宿主细胞中具有很强的逃避破坏的能力。本研究调查了单独或联合使用脂多糖（LPS）、可溶性利什曼原虫抗原（SLA）、钙离子载体（CaI）和镁（Mg2+）处理后，驻留性炎症巨噬细胞和J774细胞在体外的激活情况。单独使用LPS或与SLA和CaI联合处理的J774或炎症巨噬细胞中，一氧化氮（NO）的产生增加。相同处理对驻留巨噬细胞释放NO没有影响。对硕大利什曼原虫的摄取没有干扰，但CaI可降低寄生虫的细胞内增殖。本研究证明了CaI在减少硕大利什曼原虫在小鼠巨噬细胞内增殖方面的重要性，而Mg2+似乎增加了寄生虫的增殖。这些发现可能有助于理解宿主细胞清除这种病原体所涉及的事件。
