Le Cras Timothy D, Fernandez Lucas G, Pastura Patricia A, Laubach Victor E
Division of Pulmonary Biology, Cincinnati Children's Hospital Medical Center, 3333 Burnet Ave., Cincinnati, OH 45229-3039, USA.
J Appl Physiol (1985). 2005 Mar;98(3):1140-8. doi: 10.1152/japplphysiol.00479.2004. Epub 2004 Oct 29.
Studies in animal models have shown that, following lobectomy (LBX), there is compensatory growth in the remaining lung. The vascular growth response following right LBX (R-LBX) is poorly understood. To test the hypothesis that arterial growth and remodeling occur in response to LBX, in proportion to the amount of right lung tissue removed, two (24% of lung mass; R-LBX2 group) or three right lobes (52% of lung mass; R-LBX3 group) were removed via thoracotomy from adult rats. Sham control animals underwent thoracotomy only. Arteriograms were generated 3 wk after surgery. The areas of the left lung arteriogram, arterial branching, length of arterial branches, arterial density, and arterial-to-alveolar ratios were measured. To determine whether R-LBX causes vascular remodeling and pulmonary hypertension, muscularization of arterioles and right ventricular hypertrophy were assessed. Lung weight and volume indexes were greater in R-LBX3. Arterial area of the left lung increased 26% in R-LBX2 and 47% in R-LBX3. The length of large arteries increased in R-LBX3 and to a lesser extent in R-LBX2. The ratio of distal pulmonary arteries to alveoli was similar after R-LBX2 compared with sham but was 30% lower in R-LBX3. Muscularization of arterioles increased after R-LBX3, but not in R-LBX2. Right ventricular hypertrophy increased 50-70% in R-LBX3, but not in R-LBX2. Whereas removal of three right lung lobes induced arterial growth in the left lungs of adult rats, which was proportionate to the number of lobes removed, the ratio of distal pulmonary arteries to alveoli was not normal, and vascular remodeling and pulmonary hypertension developed.
动物模型研究表明，肺叶切除术后，剩余肺组织会出现代偿性生长。右肺叶切除（R-LBX）后的血管生长反应尚不清楚。为了验证肺叶切除后动脉生长和重塑与右肺组织切除量成比例这一假说，通过开胸手术从成年大鼠身上切除两叶（占肺质量的24%；R-LBX2组）或三叶（占肺质量的52%；R-LBX3组）右肺叶。假手术对照组动物仅接受开胸手术。术后3周进行动脉造影。测量左肺动脉造影的面积、动脉分支、动脉分支长度、动脉密度以及动脉与肺泡的比例。为了确定R-LBX是否会导致血管重塑和肺动脉高压，评估小动脉的肌化和右心室肥大情况。R-LBX3组的肺重量和体积指数更高。R-LBX2组左肺动脉面积增加了26%，R-LBX3组增加了47%。R-LBX3组大动脉长度增加，R-LBX2组增加程度较小。与假手术组相比，R-LBX2组远端肺动脉与肺泡的比例相似，但R-LBX3组低30%。R-LBX3组后小动脉肌化增加，而R-LBX2组未增加。R-LBX3组右心室肥大增加了50 - 70%，而R-LBX2组未增加。虽然切除三叶右肺叶可诱导成年大鼠左肺动脉生长，且与切除的肺叶数量成比例，但远端肺动脉与肺泡的比例不正常，并且出现了血管重塑和肺动脉高压。
